Sequences from confirmation amplicon


Confirmation sequences for line SALK_001525c and hit At1g21170 are shown.
Line ID SALK_001525c
Locus At1g21170 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:7417030
Primers used for PCR 15ea - LBb3, show primer details
   
Sequence Name 57-K110715-SALK_001525C-15ea
GenBank Accession LN485660 [GenBank]
Sequencing primer gene specific primer, 15ea: 5'-TACTTGTTGTAAAGCTCAGAGGCA-3'
Sequence (A. th genome BLAST matches underlined) 
>57-K110715-SALK_001525C-15ea
TTAGATCATCACACTGCCTGTGAGGATCAACAACACTTTCAGGAGCATTGATTGATTGTT
CTTCCTGGTGGTCATTGGAGTATCCATTTTGCCAACTCTCTCGCTTTGTAGTGCGTTGAG
AAAGGTCAGCACCAATTTGCTCCAAGTGAGCTGATATGTGCAAAAATCAATTAGCATATT
AACATAAAAATTTCTTTTTGTCTGCCCACACATTTATGTGGGCATCTATACATGTACTTT
CACGGAAGTTTGGTGTAAAAAGCTTACCAGCAAAATCCAGAAAGCAGTTCAAAAAGGCAA
GTCTAACAGATATCAGAATTTCTTCAATATGCGCAAACATATCTTCGGATCTAGCAGCTT
CACCTTTTAAAGATAGAATCATCCTGGAAAATGTATATACAGAAATTAGCAGCCATATAA
GGCAACATCGCTGTTAAAAAACAGAAATTGGTGGTAGACAATTGCATATGCAGTTCTCTG
ATTGTCTCTGAACTCCATATTTATGGTTCATTTTCTGAGTATCATAATATGACTAGTGAT
TATGGTCCGAGCAACCAGAATCATAAAAATGGCAATTTGGCAGCTTCCCATTCTTCTCTA
ACTAGAGAGAGAAGTAGAACATCAAATCCTAGGGTACGGTCTAGCTGGAATCACAGATAT
CTAATATTGTAATTATTGTGGTGTAAACAAATTGACGCTTAGACAACTTAATAACACATT
GCGGACGTTTTTAATGTACTGGGGTGGTTTTTCTTTTCACCAGTGAGACGGGCAACAGCT
GATTGCCCTTCACCGCCTGGCCCTGAGAGAGTTGCAGCAAGCGGTCCACGCTGGTTTGCC
CCAGCAGGCGAAAATCCTGTTTGATGGTGGTTC
   
Sequence Name 41-K110714-SALK_001525C-R210
GenBank Accession LN485310 [GenBank]
Sequencing primer T-DNA primer, R210: 5'-CCCAGTACATTAAAAACGTCCGC-3'
Sequence (A. th genome BLAST matches underlined) 
>41-K110714-SALK_001525C-R210
GTCATTTGTTTACCCACATAATTACATATTAGATATCTGTGATTCCAGCTAGACCGTACC
CTAGGATTTGATGTTCTACTTCTCTCTCTAGTTAGAGAAGAATGGGAAGCTGCCAAATTG
CCATTTTTATGATTCTGGTTGCTCGGACCATAATCACTAGTCATATTATGATACTCAGAA
AATGAACCATAAATATGGAGTTCAGAGACAATCAGAGAACTGCATATGCAATTGTCTACC
ACCAATTTCTGTTTTTTAACAGCGATGTTGCCTTATATGGCTGCTAATTTCTGTATATAC
ATTTTCCAGGATGATTCTATCTTTAAAAGGTGAAGCTGCTAGATCCGAAGATATGTTTGC
GCATATTGAAGAAATTCTGATATCTGTTAGACTTGCCTTTTTGAACTGCTTTCTGGATTT
TGCTGGTAAGCTTTTTACACCAAACTTCCGTGAAAGTACATGTATAGATGCCCACATAAA
TGTGTGGGCAGACAAAAAGAAATTTTTATGTTAATATGCTAATTGATTTTTGCACATATC
AGCTCACTTGGAGCAAATTGGTGCTGACCTTTCTCAACGCACTACAAAGCGAGAGAGTTG
GCAAAATGGATACTCCAATGACCACCAGGAAGAACAATCAATCAATGCTCCTGAAAGTGT
TGTTGATCCTCACAGGCAGTTGTTGATGATCTTAAGTAATATAGGTTACTGCAAAGACGA
ACTTGCCTCTGAGCTTANCAACAAGT

Last Updated on 06.12.2018 16:11