Sequences from confirmation amplicon


Confirmation sequences for line SALK_001615c and hit At3g62030 are shown.
Line ID SALK_001615c
Locus At3g62030 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:22974526
Primers used for PCR 39dh - LBb3, show primer details
   
Sequence Name 64-K109940-SALK_001615C-39dh
GenBank Accession LN485841 [GenBank]
Sequencing primer gene specific primer, 39dh: 5'-CTAACCATGAAGTCTGCACAAAAA-3'
Sequence (A. th genome BLAST matches underlined) 
>64-K109940-SALK_001615C-39dh
TTATCAAATGGGAAGGCAACATATGAAAGATCGATAACTCTATAGATGATGTGAAGAATT
GAGAATCTTTACCTTGACGGTACAAATGAAAAACTGGCTTCCATTAGTATTAGGACCAGC
GTTTGCCATGCTCAAGATTCCAGGTCCAGTATGCTTCACTGCAAATGCGGAAAACAAAAC
CATTAATACCTCAAAGAAGTAATGCCATATTTGCAGAAAACCAGAGTTTTTCTTTGTTTG
TGAGTACTTACGGGTGAAGTTTTCATCTTCGAACTTGGCACCGTAAATACTAATACCTCC
AGTACCCTGTGAGACCATATCGAAAAAGACGATTTAGAAAGAAATATCAAAATAACAAAC
AAACAGTCTGAAATGTAACTTGTTGGAAAGAACTTACATTTCCCTCGGTGAAATCACCTC
CTTACCCGTATAACACAAATCGACGCCCAAATTGTGGTGTAAACAAATTGACGCTNAGAC
AACTTAATAACACATTGCGGACGTTTTTAATGGACTGGGGTGGTTTTTCTTTTCA
   
Sequence Name 55-K109939-SALK_001615C-R210
GenBank Accession LN485620 [GenBank]
Sequencing primer T-DNA primer, R210: 5'-CCCAGTACATTAAAAACGTCCGC-3'
Sequence (A. th genome BLAST matches underlined) 
>55-K109939-SALK_001615C-R210
GTCATTTGTTACCCACAATTTGGGCGTCGATTTGTGTTATACGGGTAAGGAGGTGATTTC
ACCGAGGGAAATGTAAGTTCTTTCCAACAAGTTACATTTCAGACTGTTTGTTTGTTATTT
TGATATTTCTTTCTAAATCGTCTTTTTCGATATGGTCTCACAGGGTACTGGAGGTATTAG
TATTTACGGTGCCAAGTTCGAAGATGAAAACTTCACCCGTAAGTACTCACAAACAAAGAA
AAACTCTGGTTTTCTGCAAATATGGCATTACTTCTTTGAGGTATTAATGGTTTTGTTTTC
CGCATTTGCAGTGAAGCATACTGGACCTGGAATCTTGAGCATGGCAAACGCTGGTCCTAA
TACTAATGGAAGCCAGTTTTTCATTTGTACCGTCAAGGTAAAGATTCTCAATTCTTCACA
TCATCTATAGAGTTATCGATCTTTCATATGTTTGCCTTCCCATTTTGATTAATATTTATT
TCACAAATTTGTTTTTTTGTGCAG

Last Updated on 06.12.2018 16:11