Sequences from confirmation amplicon


Confirmation sequences for line SALK_002974c and hit At1g03050 are shown.
Line ID SALK_002974c
Locus At1g03050 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:707780
Primers used for PCR 01dx - LBb3, show primer details
   
Sequence Name 85-K110041-SALK_002974C-01dx
GenBank Accession LN486284 [GenBank]
Sequencing primer gene specific primer, 01dx: 5'-GAAACTTGAAGTGGGCTTTATTTG-3'
Sequence (A. th genome BLAST matches underlined) 
>85-K110041-SALK_002974C-01dx
TTTTTGTTTTGACTTTCTTCATTTATTCATATCAAATTAAGAAAAAGGTCTTTTACCAAA
CTCTTTTTAGTAGAATGTTAAAAACCAATATAACTTTCTTTTTTTAAAATATATAAATGA
TATGGGAAAAATTCTACGCATTGTCGGGGTCGTCGTCATCTCGTTAGTGGTAGAAAATAA
GATTTTTTTAAAAAAATCTGTCTTTGTCGTTTTTTCCGAAATTTAGCACTCATGGAGTAA
AGTTTCTAATATCCAATATAAAAATGACACGTTTCATTTTGCCATATGGTATTACAACTT
GCTTCTCCTTAGTGCTGAACCGTTGTCCGACTCGTTTTCCATTTTGAGAGACAAGGGTTT
TTCTATTTTTCACAAAACAAAATTGTGGCATTTCTGAATATAATACGAGTAAAAAATATG
TCAGCAGAAATGGGCTTGGATCGATTTTGATTTGTTGGATTCCATAATTCAATCTTGGGC
TTCCAATCCATAAGAAAAAAGCTTGGTGTATTTTTTTTTACAAATTAGAAGAAAGTTTTT
TCCTCTTTAATCAGTACTCTTCTCCTTACCCATTAATGGCAAATGCAAACTAAATTTCAG
TTGACCCGTCAAACAATAAGTTAATTTTTTGCCACGTGGCCATATTTCTCACCGGACAAA
GCCTCCAATAACCTCGGCGACTTTAGATGGTGAATAATCGACGAACAACAAAGAAACTAC
ATTGACGAAACAAACAAACAAAAACATAGAAGAAGAAGAAGAAGAAACAAAACAAAACTA
AACAAGAAACAGAGGAGAAAAAAGGAAACGACGAGGAAAAACAAAAACAAATAAAAA
   
Sequence Name 78-K110040-SALK_002974C-R210
GenBank Accession LN486185 [GenBank]
Sequencing primer T-DNA primer, R210: 5'-CCCAGTACATTAAAAACGTCCGC-3'
Sequence (A. th genome BLAST matches underlined) 
>78-K110040-SALK_002974C-R210
TCTCGCTGCGTTTGGTCCTTTACGGCTCCTATGGCTCGTTTAAACTTACTCGAACCCATT
TTTATTCTGATTAGTTAGTAAATTTGTATTAAAGACAAAAAAAAGAAAAGAAAATATGTA
AAAGAAAAGATTTGAAGAGAGAAAAGGGAAGAACGAGGCTTGTTTTTATTTTGTTTTTGT
TTTTCCTCGTCGTTTCCTTTTTTCTCCTCTGTTTCTTGTTTAGTTTTGTTTTGTTTCTTC
TTCTTCTTCTTCTATGTTTTTGTTTGTTTGTTTCGTCAATGTAGTTTCTTTGTTGTTCGT
CGATTATTCACCATCTAAAGTCGCCGAGGTTATTGGAGGCTTTGTCCGGTGAGAAATATG
GCCACGTGGCAAAAAATTAACTTATTGTTTGACGGGTCAACTGAAATTTAGTTTGCATTT
GCCATTAATGGGTAAGGAGAAGAGTACTGATTAAAGAGGAAAAAACTTTCTTCTAATTTG
TAAAAAAAAATACACCAAGCTTTTTTCTTATGGATTGGAAGCCCAAGATTGAATTATGGA
ATCCAACAAATCAAAATCGATCCAAGCCCATTTCTGCTGACATATTTTTTACTCGTATTA
TATTCAGAAATGCCACAATTTTGTTTTGTGAAAAATAGAAAAACCCTTGTCTCTCAAAAT
GGAAAACGAGTCGGACAACGGTTCAGCACTAAGGAGAAGCAAGTTGTAATACCATATGGC
AAAATGAAACGTGTCATTTTTATATTGGATATTAGAAACTTTACTCCATGAGTGCTA

Last Updated on 06.12.2018 16:11