Sequences from confirmation amplicon


Confirmation sequences for line SALK_003000c and hit At1g78110 are shown.
Line ID SALK_003000c
Locus At1g78110 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:29392903
Primers used for PCR 41us - LBb3, show primer details
   
Sequence Name 54-K117993-SALK_003000C-41us
GenBank Accession LN485610 [GenBank]
Sequencing primer gene specific primer, 41us: 5'-GAACAACAAAGATGACGACAAGAA-3'
Sequence (A. th genome BLAST matches underlined) 
>54-K117993-SALK_003000C-41us
CGATCTTGAGACCAGAGACAGAACCAGCGGTTCCGCCGCCAAACGCGCTTTTGTTGATGC
GGTGTAGATCAGCTCCAGCGAAGAGTTGGTTAGAAGAGAGAATGAAAGTAAAAACAGAGC
AAGAAAAGAGAGAAGAACAAAAAGAGGAAAAAGAAACAGAGGATCAAGAAACGAGTATGA
AGACAAAGAAGAAGGATTTGAGATCATTAATGGAAGAAGAGAAGATGGAATTGGTGTTGA
TGAGATACGATACTGAGTTTTACAGACTCTCTTCAGACATAGCTAAGGAAACTTGGGTTG
TCGGAGGAATTCAAGATCCTCTGTCTCGGAGTCGAAGCTGGAAAAATTGACACTTAGACA
ACTTAATAACACATTGCGGACGTTTTTAATGTACTGGGGTGGTTTTTCTTTTCACCAGTG
AGACGGGCAACAGCTGATTGCCCTTCACCGCCTGGCCCTGAGAGAGTTGCAGCAAGCGGT
CCACGCTGGTTTGCCCCAGCAGGCGAAAATCCTGTTTGATGGTGGTTCCGAAATCGGCAA
AAT
   
Sequence Name 43-K117992-SALK_003000C-R210
GenBank Accession LN485361 [GenBank]
Sequencing primer T-DNA primer, R210: 5'-CCCAGTACATTAAAAACGTCCGC-3'
Sequence (A. th genome BLAST matches underlined) 
>43-K117992-SALK_003000C-R210
TTTTTCCGCTTCGACTCCGAGACAGAGGATCTTGAATTCCTCCGACAACCCAAGTTTCCT
TAGCTATGTCTGAAGAGAGTCTGTAAAACTCAGTATCGTATCTCATCAACACCAATTCCA
TCTTCTCTTCTTCCATTAATGATCTCAAATCCTTCTTCTTTGTCTTCATACTCGTTTCTT
GATCCTCTGTTTCTTTTTCCTCTTTTTGTTCTTCTCTCTTTTCTTGCTCTGTTTTTACTT
TCATTCTCTCTTCTAACCAACTCTTCGCTGGAGCTGATCTACACCGCATCAACAAAAGCG
CGTTTGGCGGCGGAACCGCTGGTTCTGTCTCTGTGTCTTCAAGATCGCGTTTCTCATCAC
ACTTGTTGTTGTTCTTGTCGTCATCTNTGTTGTTC

Last Updated on 06.12.2018 16:11