Sequences from confirmation amplicon


Confirmation sequences for line SALK_003331 and hit At3g26932 are shown.
Line ID SALK_003331
Locus At3g26932 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:9930877
Primers used for PCR 42us - LBb3, show primer details
   
Sequence Name 55-K117993-SALK_003331C-42us
GenBank Accession LN485631 [GenBank]
Sequencing primer gene specific primer, 42us: 5'-AAAGACACAACCTTTAATCATGGG-3'
Sequence (A. th genome BLAST matches underlined) 
>55-K117993-SALK_003331C-42us
TATTTGGTATGACTTCTTCTCTTGGCTTCATTTCCCAGCTCCCTTGTGATGAACGGTGAT
GAATTAGGGTTAGGGTTAGGTTTTGGTGGAGGAGCTGAACAAACTGGGATCACTGATCTG
ATGTGAACTGCTGGAGCTAGTTTCTGATGGTTTAACACAAAACTTGGAAAGGGTAAGAGC
TTACTATAACAGTTTCTCTCCACTGATGATGATAATGGTGATGGTGATGATGTTTTGATT
CTCATCTCTGGCTTAGGATCATCTAGGCTCTGTTTCTTCTCTGCATATTCTTGACATTTC
TCTAGCAATGATTTGACTTTGGATTGTTCTTGTGTGAGATTTGTTCTTGAAGGCCAGAAT
CTCTGTGGTGGTGGTGATGATGATGAAGCTTCATTGGTATATAGATTGATGGATCTGAGA
CAACTTGACGCTTAGACAACTTAATAACACATTGCGGACGTTTTTAATGTACTGGGGTGG
TTTTTCTTTTCACCAGTGAGACGGGCAACAGCTGATTGCCCTTCACCGCCTGGCCCTGAG
AGAGTTGCAGCAAGCGGTCCACGCTGGTTTGCCCCAGCAGGCGAAAATCCTGTTTGATGG
TGGTTCCGAAATCGGCAAAAT
   
Sequence Name 44-K117992-SALK_003331C-R210
GenBank Accession LN485381 [GenBank]
Sequencing primer T-DNA primer, R210: 5'-CCCAGTACATTAAAAACGTCCGC-3'
Sequence (A. th genome BLAST matches underlined) 
>44-K117992-SALK_003331C-R210
ATCCATCAATCTATATACCAATGAAGCTTCATCATCATCACCACCACCACAGAGATTCTG
GCCTTCAAGAACAAATCTCACACAAGAACAATCCAAAGTCAAATCATTGCTAGAGAAATG
TCAAGAATATGCAGAGAAGAAACAGAGCCTAGATGATCCTAAGCCAGAGATGAGAATCAA
AACATCATCACCATCACCATTATCATCATCAGTGGAGAGAAACTGTTATAGTAAGCTCTT
ACCCTTTCCAAGTTTTGTGTTAAACCATCAGAAACTAGCTCCAGCAGTTCACATCAGATC
AGTGATCCCAGTTTGTTCAGCTCCTCCACCAAAACCTAACCCTAACCCTAATTCATCACC
GTTCATCACAAGGGAGCTGGGAAATGGAAGCCAAGAGAAGAAGTCATTACCAAATTAGGA
TTAGAAACAAAATCTGGATCAAACCCATGATTAAAGGTGGTGTCTTT

Last Updated on 06.12.2018 16:11