Sequences from confirmation amplicon


Confirmation sequences for line SALK_003425c and hit At1g62020 are shown.
Line ID SALK_003425c
Locus At1g62020 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:22920455
Primers used for PCR 28de - LBb3, show primer details
   
Sequence Name 01-K109680-SALK_003425C-28de
GenBank Accession LN484273 [GenBank]
Sequencing primer gene specific primer, 28de: 5'-CCAAATTAACGCAAAAGAGAGAAC-3'
Sequence (A. th genome BLAST matches underlined) 
>01-K109680-SALK_003425C-28de
ATCTATACACCTTCTTCTTCTTCTTCTTCTTCCTCTTCCTCTTCGCTTCGTTCGTAAACT
CATCTGATCTGTGGTAACCACCATTGTTCCTCTCCTTACGATTTCGAATTTCTGTTTTGA
TCTCACTACCATTGTTGCAATTTAGATCTATTTCTAATTGTTCGTGATTCGCTTTTTTTT
TTCTGTGATCTGGTTTTTGTAAAGGGCCATTATCGTTGAAAAATTATGTTGACAAAGTTC
GAAACCAAAAGTAACAAAGTTAAGGGACTGAGTTTTCACCCTAAACAACCATGGATTCTC
GCGAGTTTGCACAGGGGCGTGATCCACCTCTGGGATTATCGTATGGGTACTTTGATCGAT
AGATTCAACAAACACAAAGGACCTGTTCGGGGGGTTCATTTCCACAATTCTCAGCCTCTC
TTCGTCTCCGGAGGTATATATAATTCGAAATCATTGTTAGTGAAATCGTATAAGCTCAAA
AAAATGGATCTATACATGATTTGTTGCATTACAATGATGTTGATCTTGATATTGCAGGGG
ATGATTACAAGATTAAAGTGTGGAACTACAAAAACCACAGGTGCCTTTTCACTCTTCTAG
GGCATCTTGATTATATCCGCACGGTTCAGTTCCATCATGAGTACCCATGGATTGTGAGTG
CAAGTGATGATCAAACTATCCGTATATGGAACTGGCAATCACGGACTTGTGTTTCTGTAT
TGACTGGCCACAATCATTATGTTATGTGTGCTTCTTTCCATCCGAAAGAAGACCTCGTCG
TATCACCTTCGTTAAATCAAACTGTTCGTGTTTGGAATA
   
Sequence Name 17-K109679-SALK_003425C-R210
GenBank Accession LN484741 [GenBank]
Sequencing primer T-DNA primer, R210: 5'-CCCAGTACATTAAAAACGTCCGC-3'
Sequence (A. th genome BLAST matches underlined) 
>17-K109679-SALK_003425C-R210
GTCATTTGTTTCACCACAATATATCCTGAAGGTCATGATATCATCAGCAGGAGATACAGT
CTTCTTCCTAAGAGCACCAATATCCCAAACACGAACAGTCTGATCTAACGAAGCTGATAC
GACGAGGTCTTCTTTCGGATGGAAAGAAGCACACATAACATAATGATTGTGACCAGTCAA
TACAGAAACACAAGTCCGTGATTGCCAGTTCCATATACGGATAGTTTGATCATCACTTGC
ACTCACAATCCATGGGTACTCATGATGGAACTGAACCGTGCGGATATAATCAAGATGCCC
TAGAAGAGTGAAAAGGCACCTGTGGTTTTTGTAGTTCCACACTTTAATCTTGTAATCATC
CCCTGCAATATCAAGATCAACATCATTGTAATGCAACAAATCATGTATAGATCCATTCTT
TTGAGCTTATACGATTTCACTAACAATGATTCCGAATTATATATACCTCCGGAGACGAAG
AGAGGCTGAGAATTGTGGAAATGAACACCACGAACAGGTCCTTCGTGTTCGTCGAATCTA
TCGATCAAAGTACCCATACGATAATCCCAGAGCTGGATCACGCCACTGTGCAAACTCGCG
AGAATCCATGGTCGTTTAGGGTGAAAACTCAGTCCCTTAACTCTGTTACTTTTGGTTTCG
AACTTTGTCAACATAATCTCTCAACGATAATGGCACTCTACAAAAACCAGATCACAGAAA
AAAAAAAGCGAATCACGAACAATTAGAAATAGATCTAAATTGCAACAATGGTAGTGAGAT
CAAAACAGAAATTCGAAATCGTAAGGAGAGGAACAATGGTGGTTACCACAGATCAGATGA
GTTTACGACCGAACCGAAGAGGAAGAGGA

Last Updated on 06.12.2018 16:11