Sequences from confirmation amplicon


Confirmation sequences for line SALK_003739 and hit At2g07750 are shown.
Line ID SALK_003739
Locus At2g07750 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr2:3576951
Primers used for PCR 33wm - LBb3, show primer details
   
Sequence Name 33-K118709-SALK_003739C-33wm
GenBank Accession LN485193 [GenBank]
Sequencing primer gene specific primer, 33wm: 5'-ATTACTCATTTTCGCATCAAATCG-3'
Sequence (A. th genome BLAST matches underlined) 
>33-K118709-SALK_003739C-33wm
TTTGGTTAGGGTTTAGCTTTTCAAGTTAAACCTTTTTTCCCCCCGAAGAAGAATCAATAC
GACCGATCAAATCAATCCATTGAATCTATGGCGATGGCTATGAGACTTCCAGCGATATCT
AGAGCTGTGACGGAAGTAGCTTCGTCTCCGGTTGGTTTGAGACGACTGTTCTGTTCAAAT
GCTTCAAGGTTCTCTTTTCTGTCTCCACCGGCTCGTCGTCAGGCTGAACCTAGCACCAAT
CTCTTCCACTCCGGTAACAAACCCTTTTCATCTCTCAGTTAATTTCTGGTTCTTTCGAGG
GTTTATCGTTGTTCGAGTATATGTTTGATGCTTTTTAAAGATCCTTCCTTTATGAATTCA
GATTTAGATTATCAGGTTCGTCTAAGATGAACTATATGTGTTTAAGAATTTGATAAGTTC
AATTATTCATTTTTGGTTCTGATTTCCAGAAAAATGTCTGCTTTCGATTGCTTAACTCTA
GAAATGTATTGTAAGAGTTAAAGAGAGTTTCTTTGCACTCAATTACTTCAGAATTCGGTT
TGTTCGGTTAAAATTTACCCATTGTGGTGTAAACAAATTGACGCTTAGACAACTTAATAA
CACATTGCGGACGTTTTTAATGTACTGGGGTGGTTTTTCTTTTCACCAGTGAGACGGGCA
ACAGCTGATTGCCCTTCACCGCCTGGCCCTGAGAGAGTTGCAGCAAGCGGTCCACGCTGG
TTTGCCCCAGCAGGCGAAAATCCTGTTTGATGGTGGTTCCGAATCGGGCAA
   
Sequence Name 08-K118708-SALK_003739C-R210
GenBank Accession LN484506 [GenBank]
Sequencing primer T-DNA primer, R210: 5'-CCCAGTACATTAAAAACGTCCGC-3'
Sequence (A. th genome BLAST matches underlined) 
>08-K118708-SALK_003739C-R210
GTCATTTGTTACCCACAATGGGTAAATTTTAACCGAACAAACCGAATTCTGAAGTAATTG
AGTGCAAAGAAACTCTCTTTAACTCTTACAATACATTTCTAGAGTTAAGCAATCGAAAGC
AGACATTTTTCTGGAAATCAGAACCAAAAATGAATAATTGAACTTATCAAATTCTTAAAC
ACATATAGTTCATCTTAGACGAACCTGATAATCTAAATCTGAATTCATAAAGGAAGGATC
TTTAAAAAGCATCAAACATATACTCGAACAACGATAAACCCTCGAAAGAACCAGAAATTA
ACTGAGAGATGAAAAGGGTTTGTTACCGGAGTGGAAGAGATTGGTGCTAGGTTCAGCCTG
ACGACGAGCCGGTGGAGACAGAAAAGAGAACCTTGAAGCATTTGAACAGAACAGTCGTCT
CAAACCAACCGGAGACGAAGCTACTTCCGTCACAGCTCTAGATATCGCTGGAAGTCTCAT
AGCCATCGCCATAGATTCAATGGATTGATTTGATCGGTCGTATTGATTCTTCTTCGGGGG
GAAAAAAGGTTTTAACTTTGAAAAGCTAAAACCCTAAACCAAAAAGCAGAGAACAAACAA
AATCGATTTGATGCGAAAATGAGTAA

Last Updated on 06.12.2018 16:11