Sequences from confirmation amplicon


Confirmation sequences for line SALK_004394 and hit At5g49940 are shown.
Line ID SALK_004394
Locus At5g49940 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:20316813
Primers used for PCR 05ey - LBb3, show primer details
   
Sequence Name 76-K111509-SALK_004394C-05ey
GenBank Accession LN486149 [GenBank]
Sequencing primer gene specific primer, 05ey: 5'-TTCCTATGGCTGTCTTCTGTTTTT-3'
Sequence (A. th genome BLAST matches underlined) 
>76-K111509-SALK_004394C-05ey
TTTCACTCTAGTTTTTGTAGAATCTTGAATTTGTAATGAACTATCAGAGATTTTGATGTA
TATAGTTGGTTAGTGCTCAGAAGAACTTTCACAGATTTGAAAACCAACTGTTCTAGTTTA
GAATCAGATGCTTTTCTTGTCTTACTAGTCTCCGTGAAACTTGGGAAGCTCGAATTTGGT
GTTGTGATTGCATTGCGCTGTGACGTGACAAATTTTGTTTCATATGGCTATCATCGGTTA
TTGGGTTGCTTTAGGGGTATATAATTTCATATGTAGTTTGTGAACGAATGGTTTCTGGTC
CCATCTCTCTATCAGACATTATATGGTTACAACGTTTCCCTAAAGTTACAGTAAAGGTTC
TCCTTTCCTAAGTTTGATATCCATTTCCATTGATTTGAAACGTTATCATTGCTTCTTCTG
TGTGTATCTTCTCCTGGAGAGATATGTATTTAGGTTTTTATGGTTGATTTCTGGATTTTT
GCAGTTGTCAAGGCAGTAGCAACCCCAGACCCCATCTTGGAAGTACCTTTAACTGAGGAA
AATGTAGAAAGCGTTTTGGATGAAATCCGACCTTACCTTATGTCTGATGGTGGTAATGTG
GCATTACATGAGATCGATGGAAATATTGTGCGGGTTAAGCTGCAGGGAGCATGCGGATCA
TGTCCAAGTGAGACGCTNAGACNACTTAATAACACATTGCGGACGTTTTTAATGTACTGG
GGTGGTTTTTCTTTTCACCAGTGAGACGGGCAACAGCTGATTGCCCTTCACCGCCNGGCC
CTGAGAGAGTTGCAGCAAGCGGTCCACGCTGGTTTGCCCCAGCAGGCGAAAATCCTGTTT
GATGGTGGTTCCGAA
   
Sequence Name 62-K111508-SALK_004394C-R210
GenBank Accession LN485792 [GenBank]
Sequencing primer T-DNA primer, R210: 5'-CCCAGTACATTAAAAACGTCCGC-3'
Sequence (A. th genome BLAST matches underlined) 
>62-K111508-SALK_004394C-R210
GACATGATCCGCATGCTCCCTGCAGCTTAACCCGCACAATATTTCCATCGATCTCATGTA
ATGCCACATTACCACCATCAGACATAAGGTAAGGTCGGATTTCATCCAAAACGCTTTCTA
CATTTTCCTCAGTTAAAGGTACTTCCAAGATGGGGTCTGGGGTTGCTACTGCCTTGACAA
CTGCAAAAATCCAGAAATCAACCATAAAAACCTAAATACATATCTCTCCAGGAGAAGATA
CACACAGAAGAAGCAATGATAACGTTTCAAATCAATGGAAATGGATATCAAACTTAGGAA
AGGAGAACCTTTACTGTAACTTTAGGGAAACGTTGTAACCATATAATGTCTGATAGAGAG
ATGGGACCAGAAACCATTCGTTCACAAACTACATATGAAATTATATACCCCTAAAGCAAC
CCAATAACCGATGATAGCCATATGAAACAAAATTTGTCACGTCACAGCGCAATGCAATCA
CAACACCAAATTCGAGCTTCCCAAGTTTCACGGAGACTAGTAAGACAAGAAAAGCATCTG
ATTCTAAACTAGAACAGTTGGTTTTCAAATCTGTGAAAGTTCTTCTGAGCACTAACCAAC
TATATACATCAAAATCTCTGATAGTTCATTACAAATTCAAGATTCTAACAAAAACTAGAG
TTGAAATAATATTTCCCTAATGCAACCCAAATAAAAACAGAAGACAGCCATAGGA

Last Updated on 06.12.2018 16:11