Sequences from confirmation amplicon


Confirmation sequences for line SALK_004447 and hit At1g17750 are shown.
Line ID SALK_004447
Locus At1g17750 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:6108631
Primers used for PCR 50cs - LBb3, show primer details
   
Sequence Name 82-K109033-SALK_004447C-50cs
GenBank Accession LN486242 [GenBank]
Sequencing primer gene specific primer, 50cs: 5'-TTCATCTTGGGTTCTAATCAGCTT-3'
Sequence (A. th genome BLAST matches underlined) 
>82-K109033-SALK_004447C-50cs
AGACCCTTGAGCGAGTCAGACTTGAAGATAACAAACTTTCAGGTCTTTTAAAGGAATTCC
CTGAGAGTCTTAGTCTTTCCTATGTGACCCTCGGAAGCAATAGCTTTGAAGGATCCATCC
CGCGCAGCTTGGGAAGCTGTAAAAATCTCTTGACTATTGACCTTTCTCAAAACAAACTCA
CGGGTCTGATACCTCCAGAACTGGGAAATCTGCAAAGCCTTGGACTGTTGAACCTTTCAC
ATAATTATCTGGAAGGTCCTCTGCCATCCCAGCTATCAGGCTGTGCGAGACTTCTGTATT
TTGATGTTGGATCCAACTCATTGAACGGTTCTATTCCATCAAGCTTCAGAAGCTGGAAAA
GCTTGTCCACTTTAGTTCTCAGTGACAATAATTTTCTAGGAGCTATTCCACAGTTCTTGG
CAGAGCTTGACCGACTCTCAGATCTGCGGATAGCTCGAAATGCTTTTGGAGGTAAGATTC
CTTCCTCGGTTGGCTTGTTGAAGAGTCTACGCTATGGCTCAGACCTCAGTGCGAACGTAT
TTACGGGTGAGATTCCAACCACACTGGGGGCTCTTATCAATCTTGA
   
Sequence Name 66-K109032-SALK_004447C-R210
GenBank Accession LN485892 [GenBank]
Sequencing primer T-DNA primer, R210: 5'-CCCAGTACATTAAAAACGTCCGC-3'
Sequence (A. th genome BLAST matches underlined) 
>66-K109032-SALK_004447C-R210
GTCATTTGTTTCACCACAATAAGCGTTAATGTTGAGACGTTCAAGATTGATAAGAGCCCC
CAGTGTGGTTGGAATCTCACCCGTAAATACGTTCGCACTGAGGTCTAAGCCATAGCGTAG
ACTCTTCAACAAGCCAACCGAGGAAGGAATCTTACCTCCAAAAGCATTTCGAGCTATCCG
CAGATCTGAGAGTCGGTCAAGCTCTGCCAAGAACTGTGGAATAGCTCCTAGAAAATTATT
GTCACTGAGAACTAAAGTGGACAAGCTTTTCCAGCTTCTGAAGCTTGATGGAATAGAACC
GTTCAATGAGTTGGATCCAACATCAAAATACAGAAGTCTCGCACAGCCTGATAGCTGGGA
TGGCAGAGGACCTTCCAGATAATTATGTGAAAGGTTCAACAGTCCAAGGCTTTGCAGATT
TCCCAGTTCTGGAGGTATCAGACCCGTGAGTTTGTTTTGAGAAAGGTCAATAGTCAAGAG
ATTTTTACAGCTTCCCAAGCTGCGCGGGATGGATCCTTCAAAGCTATTGCTTCCGAGGTT
CACATAGGAAAGACTAAGACTCTCAGGGAATTCCGGAAGAACACCTGAAAGTTTGTTATC
TTCAAGTCTGACTCGCTCAAGGGTCTTACACTGACGAATAGACGCTGGTATCTTACCATG
AAGCTGATTAGAACCCAAGATG

Last Updated on 06.12.2018 16:11