Sequences from confirmation amplicon


Confirmation sequences for line SALK_004733c and hit At4g24190 are shown.
Line ID SALK_004733c
Locus At4g24190 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr4:12554825
Primers used for PCR 11jg - LBb3, show primer details
   
Sequence Name 05-K113376-SALK_004733C-11jg
GenBank Accession LN484402 [GenBank]
Sequencing primer gene specific primer, 11jg: 5'-AGTGTCTGTGTTGGTGATTTTCAG-3'
Sequence (A. th genome BLAST matches underlined) 
>05-K113376-SALK_004733C-11jg
AGACGTTTTGGGTGAGGTGACACTGCTAAGCTTGAGATCCAGGTTAGCTACATGTTTCTC
AATATGATTTTTATGCTTGAGAGTGTTGAAAATAGAGTTTCATCGATTTGTTTTATCAAA
TGCTGATCTCAGTAAATTTAGCCTTTCGTTGGAATTGTGTCTAGTGTAAGCTTTTTTCTT
ACCTCCGTAACTATGCTATGGTATGCAGATTAAGCTAGACAAAGCCAAGAAGATTCTTTC
TATTCGAGACCGGGGAATTGGTATGACTAAGGAAGACTTAATCAAGAACCTTGGTACCAT
AGCAAAATCTGGAACGTCAGGTACGTTACTTATGTGGTGATACGTTGTCCTGCATAATAG
TATGGATCAATAGTATTGTGGTGTAAACAAATTGACGCTTAGACAACTTAATAACACATT
GCGGACGTTTTTAATGTACTGGGGTGGTTTTTCTTTTCACCAGTGAGACGGGCAACAGCT
GATTGCCCTTCACCGCCTGGCCCTGAGAGAGTTGCAGCAAGCGGTCCACGCTGGTTTGCC
CCAGCAGGCGAAAATCCTGTTTGATGGTGGTTCCGAATCNGGCAAAA
   
Sequence Name 01-K113375-SALK_004733C-R210
GenBank Accession LN484284 [GenBank]
Sequencing primer T-DNA primer, R210: 5'-CCCAGTACATTAAAAACGTCCGC-3'
Sequence (A. th genome BLAST matches underlined) 
>01-K113375-SALK_004733C-R210
TTTGTTTACCACAATACTATTGATCCATACTATTATGCAGGACAACGTATCACCTTATAA
GTACGTACCTGACGTTCCAGATTTTGCTATGGTACCAAGGTTCTTGATTAAGTCTTCCTT
AGTCATACCAATTCCCCGGTCTCGAATAGAAAGAATCTTCTTGGCTTTGTCTAGCTTAAT
CTGCATACCATAGCATAGTTACGGAGGTAAGAAAAAAGCTTACACTAGACACAATTCCAA
CGAAAGGCTAAATTTACTGAGATCAGCATTTGATAAAACAAATCGATGAAACTCTATTTT
CAACACTCTCAAGCATAAAAATCATATTGAGAAACATGTAGCTAACCTGGATCTCAAGCT
TAGCAGTGTCACCTTCACCCAAAACGTCTTTGTCAGTGAGTGCAAGGAACCTAATTTTGT
CCAAGGCCTGAAAATCACCAACACAGACAC

Last Updated on 06.12.2018 16:11