Sequences from confirmation amplicon


Confirmation sequences for line SALK_005045 and hit At3g45630 are shown.
Line ID SALK_005045
Locus At3g45630 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:16749296
Primers used for PCR 07tw - LBb3, show primer details
   
Sequence Name 12-K116922-SALK_005045C-07tw
GenBank Accession LN484623 [GenBank]
Sequencing primer gene specific primer, 07tw: 5'-GAGTGATCATAGGGCCAGTACTCT-3'
Sequence (A. th genome BLAST matches underlined) 
>12-K116922-SALK_005045C-07tw
CTAGGTTCTCTTTTGCTCGGCATGAAGAATCAAATAGTCAAGCGTATGACAATAGAAGTT
ACAGCATATATGGGCAGTTGTCAAGAGATCAGCCTCTTCAAGAGTTTGGAGCGAACCGTG
ATATGTATCAGGATAAGCTTGGCAGTCAAAATGGTTTTGCCTCAAACTATTCCGGTGGAT
ATGAACAATTTGCTACTAGTCCTGGATTATCTTCTTACAAGAGTCCTGGTTAGTATATAT
CATAGCTTCCTATAGTTGTTTTTCGCAATTAAAATTGGTTTAACCAACTAGTATTTCATC
AAACTGTCGTTTCCTCTGCTTTTCTTAACTTTTCACCATTGTCCTCTTGTAGTGGCGAGG
ACTCAAGTTTCTGCTCCTCCCGGATTTTCTGCTCCCAACAGGTTACCTCCTCCCGGTTTC
TCTTCACATCAAAGAGGCGACCTGTCTTCTGATATTGCATCAGGTATTATATGTTACATT
CCCAAAAAAAGGATAGTTTCAACTTTCCATAAGCTGAAACTCCTGTAACACATACTTTGC
AATTTGCAGGAACTCGTTTACTTGACTCAGCCAATTTGTTGAGGAACGCATATCGGACAA
ATTGACGCTTAGACAACTTAATAACACATTGCGGACGTTTTTAATGTACTGGGGTGGTTT
TTCTTTTCACCAGTGAGACGGGCAACAGCTGATTGCCCTTCACCGCCTGGCCCTGAGAGA
GTTGCAGCAAGCGGTCCACGCTGGTTTGCCCCAGCAGGCGAAAATCCTGTTTGATGGTGG
TTCCG
   
Sequence Name 01-K116921-SALK_005045C-R210
GenBank Accession LN484289 [GenBank]
Sequencing primer T-DNA primer, R210: 5'-CCCAGTACATTAAAAACGTCCGC-3'
Sequence (A. th genome BLAST matches underlined) 
>01-K116921-SALK_005045C-R210
CGATATGCGTTCCTCACAAATTGGCTGAGTCAAGTAAACGAGTTCCTGCAAATTGCAAAG
TATGTGTTACAGGAGTTTCAGCTTATGGAAAGTTGAAACTATCCTTTTTTTGGGAATGTA
ACATATAATACCTGATGCAATATCAGAAGACAGGTCGCCTCTTTGATGTGAAGAGAAACC
GGGAGGAGGTAACCTGTTGGGAGCAGAAAATCCGGGAGGAGCAGAAACTTGAGTCCTCGC
CACTACAAGAGGACAATGGTGAAAAGTTAAGAAAAGCAGAGGAAACGACAGTTTGATGAA
ATACTAGTTGGTTAAACCAATTTTAATTGCGAAAAACAACTATAGGAAGCTATGATATAT
ACTAACCAGGACTCTTGTAAGAAGATAATCCAGGACTAGTAGCAAATTGTTCATATCCAC
CGGAATAGTTTGAGGCAAAACCATTTTGACTGCCAAGCTTATCCTGATACATATCACGGT
TCGCTCCAAACTCTTGAAGAGGCTGATCTCTTGACAACTGCCCATATATGCTGTAACTTC
TATTGTCATACGCTTGACTATTTGATTCTTCATGCCGAGCAAAAGAGAACCTAGACTGGT
CATTATGTTGCTTTAGGAGGTTAGAAGATTCCAGAGTACTGGCCCTAGGATCACTC

Last Updated on 06.12.2018 16:11