Sequences from confirmation amplicon


Confirmation sequences for line SALK_005743 and hit At2g25670 are shown.
Line ID SALK_005743
Locus At2g25670 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr2:10930116
Primers used for PCR 77sm - LBb3, show primer details
   
Sequence Name 37-K116586-SALK_005743C-77sm
GenBank Accession LN485255 [GenBank]
Sequencing primer gene specific primer, 77sm: 5'-TCTGTCGAGACTTCTAGGTGTGAA-3'
Sequence (A. th genome BLAST matches underlined) 
>37-K116586-SALK_005743C-77sm
TTTAGCTGAACGTTGTAAGATGGCTGGAGGTGGAAACAGGAGAGATGAAGGATCGATCAC
GATCCAGAGCACTAATTTGTTTGCAGCATTGGACACTCGTAAGAAGAAGAAGAAGTCTGA
CAAGGCAGGGAAGAGCAAAGGGTCTTCTAAGTCACGAGAGCCAGAGAAGGAGCCTGAGCC
TCAGGTCTATTGGGCTCCTACGCCTTTGAAAGTTAAGTCTTGGGCTGATATTGATGATGA
AGACGACGATGATGACTATTATGCAACCACTGCTCCTCCTCAGTCTGGTTGGAGTACTTC
TCTGCCTTCCCATACTGATTCCAAAGACGTTCATGTTGAGGTCAGAAGTCTAAACATTTT
ATGATTTTGTTGCGATTCCTAAATTGGTTGAGAATGGATATGATGGTTCTGTGTCTATTG
GTTAACGATTCTCTTTGTGACTGGGAATTCTGAGAGCTGGTTTCTGTTCTAATGGTAATT
TATCTTATGCTTATGGCTCTAGTTAGTTATTACAAAAATTGTTT
   
Sequence Name 20-K116585-SALK_005743C-R210
GenBank Accession LN484861 [GenBank]
Sequencing primer T-DNA primer, R210: 5'-CCCAGTACATTAAAAACGTCCGC-3'
Sequence (A. th genome BLAST matches underlined) 
>20-K116585-SALK_005743C-R210
CGCAATTTGTTACAACAATTTTTGTAATAACTAACTAGAGCCATAAGCATAAGATAAATT
AACATTAGAACAGAAACCAGCTCTCAGAATTCCCAGTCACAAAGAGAATCGTTAACCAAT
AGACACAGAACCATCATATCCATTCTCAACCAATTTAGGAATCGCAACAAAATCATAAAA
TGTTTAGACTTCTGACCTCAACATGAACGTCTTTGGAATCAGTATGGGAAGGCAGAGAAG
TACTCCAACCAGACTGAGGAGGAGCAGTGGTTGCATAATAGTCATCATCGTCGTCTTCAT
CATCAATATCAGCCCAAGACTTAACTTTCAAAGGCGTAGGAGCCCAATAGACCTGAGGCT
CAGGCTCCTTCTCTGGCTCTCGTGACTTAGAAGACCCTTTGCTCTTCCCTGCCTTGTCAG
ACTTCTTCTTCTTCTTACGAGTGTCCAATGCTGCAAACAAATTAGTGCTCTGGATCGTGA
TCGATCCTTCATCTCTCCTGTTTCCACCTCCAGCCATCTTAACAACGATTCAGCTAAAAC
CAAGATCGATATAAACCAAACAAGCTTTGATTTCACACCTA

Last Updated on 06.12.2018 16:11