Sequences from confirmation amplicon


Confirmation sequences for line SALK_005849c and hit At5g26740 are shown.
Line ID SALK_005849c
Locus At5g26740 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:9292946
Primers used for PCR 16ea - LBb3, show primer details
   
Sequence Name 25-K110755-SALK_005849C-16ea
GenBank Accession LN485000 [GenBank]
Sequencing primer gene specific primer, 16ea: 5'-TTCCCTTTAGCATAAAGCACAAGT-3'
Sequence (A. th genome BLAST matches underlined) 
>25-K110755-SALK_005849C-16ea
CAATTGCAGACAACCCTGCTTGCATCGTCGAATAAATCGCCTGCATGAAAAAGACCATAG
TGCATGTGTCAATAGATTTCAGCTAAGAGGACTGTCTTATAAAAACGCAAGATAAATAGA
ATCATCATGAGACCTCGAAATCCAAACAGAACACTCAAATGTCCAGGTCATTAATATTGT
ATGACTTGCACAGAAAAATCATCATCCAAAATGATAGGAAATACCAGGAAAGATTATGTA
AACACTGCCTTATTGATAAGTAGAAGAATTTTGTTTCATTCTGCGAACTTACCCGTCCAG
TGTTAAAGGCGGAAAGCAACATGTCATAAGAGACCATGATGGCTTTAAAGAACGACCACT
CAAACTAAGCACTACTGATCCTGGGCCTCCAACCCATGCCAAACACAGCGATAGAAAATT
GTAGATAACCCATGCTTCATAACACATTGCGGACGTTTTTAATGTACTGGGGTGGTTTTT
CTTTTCACCAGTGAGACGGGCAACAGCTGATTGCCCTTCACCGCCTGGCCCTGAGAGAGT
TGCAGCAAGCGGTCCACGCTGGTTTGCCCCAGCAGGCGAAAATCCTGTTTGATGGTGGTT
CCGAATCNGGCAAAA
   
Sequence Name 17-K110754-SALK_005849C-R210
GenBank Accession LN484743 [GenBank]
Sequencing primer T-DNA primer, R210: 5'-CCCAGTACATTAAAAACGTCCGC-3'
Sequence (A. th genome BLAST matches underlined) 
>17-K110754-SALK_005849C-R210
ATTTTCATCGCTGTGTTTGGCATGGGTTGGAGGCCCAGGATCAGTAGTGCTTATTTTGAG
TGGTCGTTCTTTAAAGCCATCATGGTCTCTTATGACATGTTGCTTTCCGCCTTTAACACT
GGACGGGTAAGTTCGCAGAATGAAACAAAATTCTTCTACTTATCAATAAGGCAGTGTTTA
CATAATCTTTCCTGGTATTTCCTATCATTTTGGATGATGATTTTTCTGTGCAAGTCATAC
AATATTAATGACCTGGACATTTGAGTGTTCTGTTTGGATTTCGAGGTCTCATGATGATTC
TATTTATCTTGCGTTTTTATAAGACAGTCCTCTTAGCTGAAATCTATTGACACATGCACT
ATGGTCTTTTTCATGCAGGCGATTTATTCGACGATGCAAGCAGGGTTGTCTGCAATTTGT
AATTCTGAAGCCTATCCTAGTTGCTGTCACACTTGTGCTTTATGCTAAAGGGA

Last Updated on 06.12.2018 16:11