Sequences from confirmation amplicon


Confirmation sequences for line SALK_006148c and hit At3g10090 are shown.
Line ID SALK_006148c
Locus At3g10090 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:3109597
Primers used for PCR 17ay - LBb3, show primer details
   
Sequence Name 53-K107479-SALK_006148C-17ay
GenBank Accession LN485574 [GenBank]
Sequencing primer gene specific primer, 17ay: 5'-GAAACCAGTTAATTATTGGTGCGT-3'
Sequence (A. th genome BLAST matches underlined) 
>53-K107479-SALK_006148C-17ay
TCTAAACTCTTATCGCCGCTAAACTCTCTACCCATCGCAGAGCCTCTCTAAAGGTTATCT
TCATGATCTCACTCAATACATATGTTTCGTTACTATATGTTTTTGCTTCTGATTGTCGTT
ATTCTGTAATCGTTTAGAGTCGTTTATGGTTTCCCCCATTTTGTCTGTAATGTCCATGCT
CTGTTTCTTTACAGTTTCCTCTCCGGCTTGTTAGATATCAGTTTAGTTCCTGATTTACTG
AGAAACCAAGCTTTTGAGCTTAAGTATGTTAGGGTTCTGACGTTGATTGAGAAATATTAT
AAACTGGTTTTCAAGTTTATTTCTTTCTGGAATTTCTTTTGTAGTCTGATTATAAGGTAC
ACTGCTAGATATACTAGATATATTGTGGTGTAAACAAATTGACGCTTAGACAACTTAATA
ACACATTGCGGACGTTTTTAATGTACTGGGGTGGTTTTTCTTTTCACCAGTGAGACGGGC
AACAGCTGATTGCCCTTCACCGCCTGGCCCTGAGAGAGTTGCAGCAAGCGGTCCACGCTG
GTTTGCCCCAGCAGGCGAAAATCCTGTTTGATGGTGGTTCCGAATCCGGCAAAA
   
Sequence Name 69-K107480-SALK_006148C-LBb3
GenBank Accession LN485971 [GenBank]
Sequencing primer T-DNA primer, LBb3: 5'-ATTTTGCCGATTTCGGAAC-3'
Sequence (A. th genome BLAST matches underlined) 
>69-K107480-SALK_006148C-LBb3
GGGCAACCAGCGTGGACCGCTTGCTGCAACTCTCTCAGGGCCAGGCGGTGAAGGGCAATC
AGCTGTTGCCCGTCTCACTGGTGAAAAGAAAAACCACCCCAGTACATTAAAAACGTCCGC
AATGTGTTATTAAGTTGTCTAAGCGTCAATTTGTTTACACCACAATATATCTAGTATATC
TAGCAGTGTACCTTATAATCAGACTACAAAAGAAATTCCAGAAAGAAATAAACTTGAAAA
CCAGTTTATAATATTTCTCAATCAACGTCAGAACCCTAACATACTTAAGCTCAAAAGCTT
GGTTTCTCAGTAAATCAGGAACTAAACTGATATCTAACAAGCCGGAGAGGAAACTGTAAA
GAAACAGAGCATGGACATTACAGACAAAATGGGGGAAACCATAAACGACTCTAAACGATT
ACAGAATAACGACAATCAGAAGCAAAAACATATAGTAACGAAACATATGTATTGAGTGAG
ATCATGAAGATAACCTTTAGAGAGGCTCTGCGATGGGTAGAGAGTTTTAGCGGCGATAAG
AGTTTTAAGAGCAGAGATCCTAGAACCCTAATTCGAGAGACGCACCAATAATACNTGGGT
TT

Last Updated on 06.12.2018 16:11