Sequences from confirmation amplicon


Confirmation sequences for line SALK_006410c and hit At1g20580 are shown.
Line ID SALK_006410c
Locus At1g20580 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:7130092
Primers used for PCR 45cy - LBb3, show primer details
   
Sequence Name 46-K109566-SALK_006410C-45cy
GenBank Accession LN485410 [GenBank]
Sequencing primer gene specific primer, 45cy: 5'-GCTTACATGTTGAGCGTAAAATCA-3'
Sequence (A. th genome BLAST matches underlined) 
>46-K109566-SALK_006410C-45cy
GCCCGGGCCCGGCAGCCGGCTTTAAGATAATATCACAAAGATTCAAGAATCTAACCATTA
GTAGTGACAAAGAAGAAACAAACCATTAAGGGATAACAAGGATTTGAACATCTTAACAAG
TTCCATAAAACTTCCTAACATTGCATTCATGAGCACTAAATCATAAGAGAGAGAGGGTGA
ACTTACTTTCCCTCGCATTGCACCTCTACCTCTGCCAACACCCAGTGATGAGCTCTTAAC
AAATTGACGCTTAGACAACTTAATAACACATTGCGGACGTTTTTAATGTACTGGGGTGGT
TTTTCTTTTCACCAGTGAGACGGGCAACAGCTGATTGCCCTTCACCGCCTGGCCCTGAGA
GAGTTGCAGCAAGCGGTCCACGCTGGTTTGCCCCAGCAGGCGAAAATCCTGTTTGATGGT
GGTTCCGAAATCGGCAAAA
   
Sequence Name 37-K109565-SALK_006410C-R210
GenBank Accession LN485247 [GenBank]
Sequencing primer T-DNA primer, R210: 5'-CCCAGTACATTAAAAACGTCCGC-3'
Sequence (A. th genome BLAST matches underlined) 
>37-K109565-SALK_006410C-R210
GTCATTTGTTAGAGCTCATCACTGGGTGTTGGCAGAGGTAGAGGTGCAATGCGAGGGAAA
GTAAGTTCACCCTCTCTCTCTTATGATTTAGTGCTCATGAATGCAATGTTAGGAAGTTTT
ATGGAACTTGTTAAGATGTTCAAATCCTTGTTATCCCTTAATGGTTTGTTTCTTCTTTGT
CACTACTAATGGTTAGATTCTTGAATCTTTGTGATATTATCTTAAAGCCGGCTGCCGGGC
CCGGGCGTGGAACTGGAGGAAGGGGAGCGGTACCACCTGTGAGGAGATGATTGATTTTAC
GCTCAACATGTAAG

Last Updated on 06.12.2018 16:11