Sequences from confirmation amplicon


Confirmation sequences for line SALK_006564c and hit At4g26120 are shown.
Line ID SALK_006564c
Locus At4g26120 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr4:13237860
Primers used for PCR 02bt - LBb3, show primer details
   
Sequence Name 26-K108287-SALK_006564C-02bt
GenBank Accession LN485024 [GenBank]
Sequencing primer gene specific primer, 02bt: 5'-TGGTGGAGGTTCTTTATCTGTCTT-3'
Sequence (A. th genome BLAST matches underlined) 
>26-K108287-SALK_006564C-02bt
TCTGTTGAGGTAAACACAATCCACTAAATTCTCATTGACTCATAACATCATCTTTAGTCT
CCTCTGTTTTCATTTCAGAGGCAGTTCTTGGAAATTGTAGACAAAGTTGTAGTCGAAGAC
ATCTTGGTTATATTCAAGCTTGATACTCTATGTGGTACAACATACAAGAAGCTTTTGGAT
AGATGCATAGAAATTATCGTGAAGTCTGATATAGAACTAGTTAGTCTTGAGAAGTCTTTA
CCTCAACACATTTTCAAGCAAATCATAGACATCCGCGAAGCGCTCTGTCTAGAGCCACCT
AAACTAGAAAGGCATGTCAAGAACATATACAAGGCGCTAGACTCAGATGATGTTGAGCTT
GTCAAGATGCTTTTGCTAGAAGGACACACCAATCTCGATGAGGCGTATGCTCTTCATTTT
GCTATCGCTCACTGCGCTGTGAAGACCGCGTATGATCTCCTCGAGCTTGAGCTTGCGGAT
GTTAACCTTAGAAATCCGAGGGGATACACTGTGCTTCATGTTGCTGCGATGCGGAAGGAG
CCGAAGTTGATAATATCTTTGTTAATGAAAGGGGCAAATATTTTAGACACAACATTGGAT
GGTAGAACCGCTTTAGTGATTGTAAAACGACTCACTAAAGCGGATGACTACAAAACTAGT
ACGGAGGACGGTACGCCTTCTCTGAAAGGCGGATTATGCATAGAGGTACTTGAGCATGAA
CAAAAACTAGAATATTTGTCGCCTATAGAGGCTTCACTTTCTCTTCCAGTAACTCCAGAG
GAGTTGAGGATGAGGTTGCTCTATTATGAAAACCGAGGTATGCTTTCTTCCTTCACTTGA
ATATCGAATTTCGGGTAGGAAAATGACGCTTAGACAACTTAATAACACATTGCGGACGTT
TTTTATGTACTGGGGTGGTTTTTCTTTTCACCGTGAGACGGGCAACAGCTGATTTGCCTT
CACCGCCTGGCCTGAGAGAGTGCCACAAGCGGTCCCGCTGGTTTGCCCGCAAGCGAAATC
CTGTTTGATGGTGGTCGAAAT
   
Sequence Name 10-K108286-SALK_006564C-R210
GenBank Accession LN484541 [GenBank]
Sequencing primer T-DNA primer, R210: 5'-CCCAGTACATTAAAAACGTCCGC-3'
Sequence (A. th genome BLAST matches underlined) 
>10-K108286-SALK_006564C-R210
TTTTCCTCCCGAATTCGATATTCAGTGAAGGAAGAAAGCATACCTCGGTTTTCTTTATAG
AGCAACCTCATCCTCAACTCCTCTGGAGTTACTGGAAGAGAAAGTGAAGCCTCTATAGGC
GACAAATATTCTAGTTTTTGTTCATGCTCAAGTACCTCTATGCATAATCCGCCTTTCAGA
GAAGGCGTACCGTCCTCCGTACTAGTTTTGTAGTCATCCGCTTTAGTGAGTCGTTTTACA
ATCACTAAAGCGGTTCTACCATCCAATGTTGTGTCTAAAATATTTGCCCCTTTCATTAAC
AAAGATATTATCAACTTCGGCTCCTTCCGCATCGCAGCAACATGAAGCACAGTGTATCCC
CTCGGATTTCTAAGGTTAACATCCGCAAGCTCAAGCTCGAGGAGATCATACGCGGTCTTC
ACAGCGCAGTGAGCGATAGCAAAATGAAGAGCATACGCCTCATCGAGATTGGTGTGTCCT
TCTAGCAAAAGCATCTTGACAAGCTCAACATCATCTGAGTCTAGCGCCTTGTATATGTTC
TTGACATGCCTTTCTAGTTTAGGTGGCTCTAGACAGAGCGCTTCGCGGATGTCTATGATT
TGCTTGAAAATGTGTTGAGGTAAAGACTTCTCAAGACTAACTAGTTCTATATCAGACTTC
ACGATAATTTCTATGCATCTATCCAAAAGCTTCTTGTATGTTGTACCACATAGAGTATCA
AGCTTGAATATAACCAAGATGTCTTCGACTACAACTTTGTCTACAATTTCCAAGAACTGC
CTCTGAAATGAAAACAGAGGAGACTTAAGATGATGTTATGAGTCAATGAGAATTTAGTGG
ATTGTGTTTTACCTCATACAGAGTAACTAATTCTTGAATCTGGAAAACGAAAGACAGATA
AAGA

Last Updated on 06.12.2018 16:11