Sequences from confirmation amplicon


Confirmation sequences for line SALK_007134c and hit At1g71230 are shown.
Line ID SALK_007134c
Locus At1g71230 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:26853061
Primers used for PCR 22di - LBb3, show primer details
   
Sequence Name 52-K110195-SALK_007134C-22di
GenBank Accession LN485556 [GenBank]
Sequencing primer gene specific primer, 22di: 5'-GAATGATTTAGGCCATATCCAAGA-3'
Sequence (A. th genome BLAST matches underlined) 
>52-K110195-SALK_007134C-22di
TTGTCAGCACATAGTCTCCATTACCCAGCAGTGGAGAAGAAGAAAGAGTGTTCACCCAGT
ACTTGTTCCATAGTAGATCCAGAAGGTGAGAATCAAGAGATGACTTGAAATAAGTGACAT
CTAATGAATAGTACTGCCAAAACCAAAAGCCAAACATAAGAATCATGTTGGGACCTTGCA
CCGAGAAAAATCAAGAAAATGTACCTGTTTGCAGTGAACACCAAAGTCCTCAATCTTATT
TAAAGGAATAGTTTGATACTCAGAAACAGGTTCATCTGGAGGCTTATATCCTTTAGAGTA
TGTTCTGAAAGCACCAATCTCAACCTTACCAGCTGAAACAGTCCTTGTGGGATCAATAAC
AACAGCTAAAAATGGCTCCTGATGCTGTTGGTTAAGCCTCTGCGTAGAAACATCAATACC
GGAGAGCCAGCATCCATATCCAGGGTGAGAGTGATACCATCCAACAACATTCTCCAGCCG
CCCCGAAGAATGTATTGACGCTTGCTATATTGTGGTGTAAACAAATTGACGCTTAGACAA
CTTAATAACACATTGCGGACGTTTTTAATGTACTGGGGTGGTTTTTCTTTTCACCAGTGA
GACGGGCAACAGCTGATTGCCCTTCACCGCCTGGCCCTGAGAGAGTTGCAGCAAGCGGTC
CACGCTGGTTTGCCCCAGCAGGCGAAAATCCTGTTTGATGGTGGTTC
   
Sequence Name 44-K110194-SALK_007134C-R210
GenBank Accession LN485370 [GenBank]
Sequencing primer T-DNA primer, R210: 5'-CCCAGTACATTAAAAACGTCCGC-3'
Sequence (A. th genome BLAST matches underlined) 
>44-K110194-SALK_007134C-R210
GTCATTTGTTACCCACAATATAGCAAGCGTCAATACATTCTTCGGGGCGGCTGGAGAATG
TTGTTGGATGGTATCACTCTCACCCTGGATATGGATGCTGGCTCTCCGGTATTGATGTTT
CTACGCAGAGGCTTAACCAACAGCATCAGGAGCCATTTTTAGCTGTTGTTATTGATCCCA
CAAGGACTGTTTCAGCTGGTAAGGTTGAGATTGGTGCTTTCAGAACATACTCTAAAGGAT
ATAAGCCTCCAGATGAACCTGTTTCTGAGTATCAAACTATTCCTTTAAATAAGATTGAGG
ACTTTGGTGTTCACTGCAAACAGGTACATTTTCTTGATTTTTCTCGGTGCAAGGTCCCAA
CATGATTCTTATGTTTGGCTTTTGGTTTTGGCAGTACTATTCATTAGATGTCACTTATTT
CAAGTCATCTCTTGATTCTCACCTTCTGGATCTACTATGGAACAAGTACTGGGTGAACAC
TCTTTCTTCTTCTCCACTGCTGGGTAATGGAGACTATGTTGCTGGACAAATATCAGACTT
AGGTAAACTCTTGGATATGGCCTAAATCATT

Last Updated on 06.12.2018 16:11