Sequences from confirmation amplicon


Confirmation sequences for line SALK_007247c and hit At1g20803 are shown.
Line ID SALK_007247c
Locus At1g20803 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr:
Primers used for PCR BL10 - LBb3, show primer details
   
Sequence Name 73-K119131-SALK_007247C-BL10
GenBank Accession LN486094 [GenBank]
Sequencing primer gene specific primer, BL10: 5'-CTTTACACAAATGTTGGGACAATG-3'
Sequence (A. th genome BLAST matches underlined) 
>73-K119131-SALK_007247C-BL10
ACTCAAAAGTATACTCCAGGCTGCAACAACATACATGATTTTATTATCTCTTAAACCCGA
AACAAGCATTATACTACATTTTGTAATTAGTTTTCAGCAAAAAACGGAGCTTTCCTCATA
TACAATTTGTAGTAGGTTCTCACCGGTATCTCATTCATTCCTTTCTGACCATAACCAGCT
TCAGGAGGAACAATCACCGTTCTCTGCAAGAACAACATAGGTCAAAACTACTTATATGTA
ACTACATGTTGTAAGAAGAAGAGAGCGAAAATGCAGTAATGTTTCTAACCTTGCCTCCGA
CTTTCATTCCTTCGGTAATGAAATACATTGCTGGAGGAGGTTTTGGTGCAGCCTGTGCAG
AGAATAACCCATTTGGATTATCAACGAATTCACGCTTCCTTTCCTTTCCTGGCGTAGATC
CCACCTTGAACTCGTAAGGCTGCTCCAGAAAGAAGTAAGCCACCATCAAACTAAAAATTA
TGCAGAATCTAATCAAAAGGGATAGATGGTGAAGTAACCTGAGCAATACTACGGTTTCCA
GCTAAAAGCTTGGATTCTCGGGTAGAAATTGCAGTGATGCTTCTGTAACGGCAATCAAAA
TGCACCTAAATGTGGAAAATACAGACACCATTACATTGTAAAAGCGAGAACTCGATTTCG
ATATAGAGAAATTAAAATGTGAATCATTAGCTAGAGAGACCTGAGCAGTTGATCCCTCTG
TTGCTACTGGACCTTTGCCTTCCTCAATGTCATAGAAATTCAACCTTCAGCTGCAAGAAC
AGAGTAACACAAACATTGTG
   
Sequence Name 59-K119130-SALK_007247C-R210
GenBank Accession LN485727 [GenBank]
Sequencing primer T-DNA primer, R210: 5'-CCCAGTACATTAAAAACGTCCGC-3'
Sequence (A. th genome BLAST matches underlined) 
>59-K119130-SALK_007247C-R210
AACTCAATGATCCATCTGTTTTCCAAGATATCTTTCCCGTTTTTCTTGAACAAGCTCTGA
TCATATGGTGAGATTCCACTAGTCAATCTCATAACTTTGCTTATAGAGGAGACACGTTTA
TCATCATCATCATCATCGTTAACCATCGTCATCATGTCGACGTCCTCCTTCTCATCTAGT
TCCATCGAAGAGGATGCCGTCAAAGACGGTTTGGACTGACACAGGTCTCGTTTACCATCG
TACCACCGTGGAATCCAACCCATGACTCCCCACTTGGTAGCTTTAAAGTCATATCCATGA
ATCACTCCGTCGTCGTTACAGTCCTTCTTACATACAGTTTTGAAATAAGGATCGTCGGAT
ATATGCGAGTTGATAGATCGATCCGTGCATTGCATCATTGCCAGAGATTTTGGATCTAAT
TTGAAGAGAATCTCTTCAAAGATATGAAAAGGTAATCCATCCATGATTGATCTAATTAAT
AAGAAAAAGAGACAAAACAAGATTAGGGCTTTTTGCTCCGTTCCGTGATGGTGACTATTC
CAGAAGTTTTGGTGATGTTATAGTAGATATGATGTATTTATAGACGTAGATTATTTGCGC
TTTTACATATTAAAAAGGAAAGATTT
Primers used for PCR 56yn - LBb3, show primer details
   
Sequence Name 80-K121396-SALK_007247C-56yn
GenBank Accession queueing for submission to ENA/GenBank
Sequencing primer gene specific primer, 56yn: 5'-TCGATACCTCTTAGTGAGCGGATT-3'
Sequence (A. th genome BLAST matches underlined) 
>80-K121396-SALK_007247C-56yn
CCAGAGCAGAGCAGAGAAACCTAGTCTTGATATCTAAGGAATATGTGTATCTGAATGGCA
TAGAATCGCCGAAAGGGTTGCAGAAGAGAGATTTGGATCCATAGTAGGAGTTGTGTAGCA
AACCGAATCCGACCAGAGACAAGTAATTGGATTTGAAATACGGATCTTCAGATATATGCG
AGTTGATAGATCTTCTCGTGCATTTCATCATAGCTAGGGATTTGGGATCCAATTTTAATA
AAATCTTTTCGAAGACATGCAGAGGTAAGTTCTCCATGATCGAACAAGGGGAGAAAAAGA
AAAGAGAGAGGAAGAGATATTACGGTTTCTGCATAGTTCTTTGATGTGATTATTCGCTGT
AATTTGTTACTTCTAGTGTATATGTATTTATCGACGTAATTAATAATTTTTAGTCACAAA
AATCCAATAAAGAAAAGTTTGAACGTTTGTTTCGGAAACTAATATTATTCTTTTCCTTGT
TTAACTAAATGACTACGTTCTCCAAATCTCTGAGTATAAATGCAGTATCTCTTCCAAAAA
GGAAATACTACAAACCGAAAACGTTTACGTTTTGTAAGTCATTCATTATAACATTTTAAA
ACAAATTCTTCCTATTGACTGATGAGATCTAATCTACATTTTTAAGTTTTAACTCATTAT
AATATCTATTCTATTTTTGAATTTCAAACATTTCTTCATAATACATGTATCTCATCAGTC
AATAGGAAGAATTTATAGATTAAGTTATAACCGTTTACATCTTAACTAACCAGCTAGTTC
CGTGCTAAACGGTAGGAAGATCAAAGGATCTTAAACTTTTCCGGCAAATATGTTTACTAA
TAACAAGAATGATCATTAATCTGCTT
   
Sequence Name 42-K121395w-SALK_007247C-R210
GenBank Accession queueing for submission to ENA/GenBank
Sequencing primer T-DNA primer, R210: 5'-CCCAGTACATTAAAAACGTCCGC-3'
Sequence (A. th genome BLAST matches underlined) 
>42-K121395w-SALK_007247C-R210
CACAATATATCCTGAGCAATGTGTTATTAAGTTGTCTAAGCGTCAAGAAACGGAATCGAA
TATGATGATGGATGAGCCTAATTCTTCCAAGTTCCTAGACGTGGAGAGAATCAACAAAAG
AAGACGAGTCAAGTAGACAAAATTCGTATTGTGGCTTTTGGTTAATGTAATAGAAGTTTA
AGAGTTATGTTTTCCCTTTCCCTTCTTTTAAACCACCAACTTTTTTTATACTCTTTTTTT
TTTGGGAGAAGATGTTATTTATTTTACCAAAAGGGAACTTGAGGTTTACAAAAAATTTAA
AACAAACGGGCGCAAACAAAACGGTCCTTTAAAAAGACTTAGAGTTTAGGGGGAGAAGCA
AATAAAGGATCTTTCTGGTTATTAGAAAACATATTTGCCGAAAAAGTTAAAAATCCTTTG
ATCTTCCAACCGTTTAGCAGGGAACTAGCTGGTTAGTTAAAATGTTAACGGTTATAACTA
AACCTATAAATTCTCCCTATGGACGGAGGAGATACTTGTTTTATGAAAAATTGTTTGAAT
TTCAAAAATAGAATAGATTTTATATGGAGTTAAACCTTAAAAATGTAGATAAGATCTCAC
CAGTCAATAGGAAGAATTTGTTTTAAAATGTTATAAGGAATGACTTACAAAACGTA

Last Updated on 06.12.2018 16:11