Sequences from confirmation amplicon


Confirmation sequences for line SALK_007294c and hit At5g02970 are shown.
Line ID SALK_007294c
Locus At5g02970 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:697342
Primers used for PCR 23vy - LBb3, show primer details
   
Sequence Name 19-K118627-SALK_007294C-23vy
GenBank Accession LN484834 [GenBank]
Sequencing primer gene specific primer, 23vy: 5'-CTCCTCCATTGTAGATCACAAACA-3'
Sequence (A. th genome BLAST matches underlined) 
>19-K118627-SALK_007294C-23vy
CATATCTGCTAACTCTGTTTTCATCTTTCAGGATAAACTTTTAATCAAAGATCCAACGTT
TCAAGAAGTTTATCAAAGGAACGTGGAGGAATCAGTCCGTCAAGGAATCACAAAACCATT
TGTAGAAGAAGCCGTGCTTCAAGTATCGAATTGGGGCTTTACTCTTTCGGAATTCCGCAC
ACAGAAGAAATGTGCAACCAACGGTGTCCTTTCTTGGCTCATGTCAATGTACAGTGAAGC
CGAATGTGAACTAATCGGATTTCGAAAACCCATTCACATATGGCAGGTGAGTTCTGCACC
ATACAATGCATCAGTTAGTTTGTTTAGTAAAGTTAATCAAACCTTGATTTCTGTTTCTTT
AAAGGGAATGGAGGATCGAGTGGCTCCACCATCAATGAGTGACTACATTAGCAGAATGAT
TCCTGAAGCAACTGTACATAAGATTCGAAACGAAGGTCACTTCTCGTTCTTCTATTTCTG
CGATGAGTGCCATAGACAGATCTTCTATGCTTTGTTTGGAGAACCCAAAGGCCAGCTGAC
AACTTAATAACACATTGCGGACGTTTTTAATGTACTGGGGTGGTTTTTCTTTTCACCAGT
GAGACGGGCAACAGCTGATTGCCCTTCACCGCCTGGCCCTGAGAGAGTTGCAGCAAGCGG
TCCACGCTGGTTTGCCCCAGCAGGCGAAAATCCTGTTTGATGGTGGTTCCG
   
Sequence Name 08-K118626-SALK_007294C-R210
GenBank Accession LN484505 [GenBank]
Sequencing primer T-DNA primer, R210: 5'-CCCAGTACATTAAAAACGTCCGC-3'
Sequence (A. th genome BLAST matches underlined) 
>08-K118626-SALK_007294C-R210
CCTTGGGTTCTCAACAAGCATAGAAGATCTGTCTATGGCACTCATCGCAGAAATAGAAGA
ACGAGAAGTGACCTTCGTTTCGAATCTTATGTACAGTTGCTTCAGGAATCATTCTGCTAA
TGTAGTCACTCATTGATGGTGGAGCCACTCGATCCTCCATTCCCTTTAAAGAAACAGAAA
TCAAGGTTTGATTAACTTTACTAAACAAACTAACTGATGCATTGTATGGTGCAGAACTCA
CCTGCCATATGTGAATGGGTTTTCGAAATCCGATTAGTTCACATTCGGCTTCACTGTACA
TTGACATGAGCCAAGAAAGGACACCGTTGGTTGCACATTTCTTCTGTGTGCGGAATTCCG
AAAGAGTAAAGCCCCAATTCGATACTTGAAGCACGGCTTCTTCTACAAATGGTTTTGTGA
TTCCTTGACGGACTGATTCCTCCACGTTCCTTTGATAAACTTCTTGAAACGTTGGATCTT
TGATTAAAAGTTTATCCTGAAAGATGAAAACAGAGTTAGCAGATATGATGACTCCTTCTC
CTAGAGAACTCCTCTCTTTGTGTTTGTGATCTACATGGGAGGAG

Last Updated on 06.12.2018 16:11