Sequences from confirmation amplicon


Confirmation sequences for line SALK_007422c and hit At1g48620 are shown.
Line ID SALK_007422c
Locus At1g48620 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:17974566
Primers used for PCR AW83 - R204, show primer details
   
Sequence Name 54-K107002-SALK_007422C-R210
GenBank Accession LN485594 [GenBank]
Sequencing primer T-DNA primer, R210: 5'-CCCAGTACATTAAAAACGTCCGC-3'
Sequence (A. th genome BLAST matches underlined) 
>54-K107002-SALK_007422C-R210
GCGTCATTTGTTTACCCGACAATATATCTAATATAAATGTAATTAGATAACTAACTCATC
AATATAATAGACAAAGAAATTCTAGTGGACTTGGAAATACACAAACTCTTGAACTTTGAC
TGAATTAACTAAATTTCTGCTTAAAACAGACAAAACTTGATAAGATGTCTGTCTCAAAAG
ATAAGTTCATATGTAATTCTCACACTTCAACTGAACAATTATTGGTTGCTAAAGCGAGCC
ACCTACCAAGTTGCTCTTGATTACCAAACAAAGAGATATCTTAGATTAATAGTACACCAC
AAAACCAGATTAAGTATAGGCCAAAAGCAGAGACACGAATATAAATTAAACTCTAGAATT
TATTTAGTTAGATACTCTCTAGTCTCCACACACACGCTCGAAAGAGACAATGTTGAGAAT
GGAAAGTGACATTTATTCACAAGACAAAAGTTCCAACTTTTATTCTCTTATCTATTTGAC
TAGAAATATTCAAGTTCTCTATAATCACTGTGTAATCGCAGAGACAAAAAGGAATGATAT
TTTACCTTTCTGGGTCTTCCTACGGGTTTTCCAGTACGAGCAAAGCTTCTCATCGGCTTC
ATAGGCTTCTGNATAACA
   
Sequence Name 62-K107003-SALK_007422C-AW83
GenBank Accession LN485782 [GenBank]
Sequencing primer gene specific primer, AW83: 5'-GTGTTATCAGGAAGCCTATGAAGC-3'
Sequence (A. th genome BLAST matches underlined) 
>62-K107003-SALK_007422C-AW83
AACCCGTAGGAAGACCAGAAAGGTAAAATATCATTCCTTTTTGTCTCTGCGATTACACAG
TGATTATAGAGAACTTGAATATTTCTAGTCAAATAGATAAGAGAATAAAAGTTGGAACTT
TTGTCTTGTGAATAAATGTCACTTTCCATTCTCAACATTGTCTCTTTCGAGCGTGTGTGT
GGAGACTAGAGAGTATCTAACTAAATAAATTCTAGAGTTTAATTTATATTCGTGTCTCTG
CTTTTGGCCTATACTTAATCTGGTTTTGTGGTGTACTATTAATCTAAGATATCTCTTTGT
TTGGTAATCAAGAGCAACTTGGTAGGTGGCTCGCTTTAGCAACCAATAATTGTTCAGTTG
AAGTGTGAGAATTACATATGAACTTATCTTTTGAGACAGACATCTTATCAAGTTTTGTCT
GTTTTAAGCAGAAATTTAGTTAATTCAGTCAAAGTTCAAGAGTTTGTGTATTTCCAAGTC
CACTAGAATTTCTTTGTCTATTATATTGATGAGTTAGTTATCTAATTACATTTATATTAG
ATATATTGTCGGTGTAAACAAATTGACGCTTAGACAACTTAATAACACATTGCGGACGTT
TTTAATGTACTGGGGTGGTTTTTCTTTTCACCAGTGAGACGGGCAACAGCTGATTGCCCT
TCACCGCCTGGCCCTGAGAGAGTTGCAGCAAGCGGTCCACG
Primers used for PCR AW84 - LBb3, show primer details
   
Sequence Name 79-K118526-SALK_007422C-AW84
GenBank Accession LN486214 [GenBank]
Sequencing primer gene specific primer, AW84: 5'-GTTTGTTGTCTCTGCTATCCCTTC-3'
Sequence (A. th genome BLAST matches underlined) 
>79-K118526-SALK_007422C-AW84
TTTCACTTCCTCCTATCTCGGATTTCAACACAATTACAATATCCTTAGCTCTCGCTTGCT
GCATAAATGAGATATCAAAACCAAGCATAAGCAATTAAGTATCAAAACCAAGGACGGACC
ATATTGTGTTTCTGTTCATTAGATTACTTACAAACAACTCAAACTTCTTCTTCAGTTCTC
CATAGTCACCATCTTGTCGTCCAGAAGCTCCCACTGACACCGCATATATTGTGGTGTAAA
CAAATTGACGCTTAGACAACTTAATAACACATTGCGGACGTTTTTAATGTACTGGGGTGG
TTTTTCTTTTCACCAGTGAGACGGGCAACAGCTGATTGCCCTTCACCGCCTGGCCCTGAG
AGAGTTGCAGCAAGCGGTCCACGCTGGTTTGCCCCAGCAGGCGAAAATCCTGTTTGATGG
TGGTTCCGAATCGGGCAAAAT
   
Sequence Name 69-K118525-SALK_007422C-R210
GenBank Accession LN485991 [GenBank]
Sequencing primer T-DNA primer, R210: 5'-CCCAGTACATTAAAAACGTCCGC-3'
Sequence (A. th genome BLAST matches underlined) 
>69-K118525-SALK_007422C-R210
TGTTACCCACAATATATGCGGTGTCAGTGGGAGCTTCTGGACGACAAGATGGTGACTATG
GAGAACTGAAGAAGAAGTTTGAGTTGTTTGTAAGTAATCTAATGAACAGAAACACAATAT
GGTCCGTCCTTGGTTTTGATACTTAATTGCTTATGCTTGGTTTTGATATCTCATTTATGC
AGCAAGCGAGAGCTAAGGATATTGTAATTGTGTTGAAATCCGAGATAGGAGGAAGTGGAA
ATCAAGCAGTGGTTCAAGCCATACAGGACCTGGAAGGGATAGCAGAGACAACAAAC

Last Updated on 06.12.2018 16:11