Sequences from confirmation amplicon


Confirmation sequences for line SALK_007997c and hit At2g39180 are shown.
Line ID SALK_007997c
Locus At2g39180 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr2:16344239
Primers used for PCR 61kh - LBb3, show primer details
   
Sequence Name 51-K113870-SALK_007997C-61kh
GenBank Accession LN485539 [GenBank]
Sequencing primer gene specific primer, 61kh: 5'-ATGGAGATCTTTCACAACTGGACT-3'
Sequence (A. th genome BLAST matches underlined) 
>51-K113870-SALK_007997C-61kh
AGAGGACTTGATTCTTACACAACGAAGTAGATCCTCCGATAATTCACAGAGATGTGAAAA
CGTCCAACATCTTGTTAGACGGTGAAATGTGCGCAAGAATTGCGGATTTCGGATTGGTAA
GCTCGAATGAAAGGGATTCAAGCAATAGTGATAGAGAAGGTGATGTGTATGACTTTGGTA
TTGTTCTACTTGAGATACTAAGCGGGCGAAAAGCTATCGATAGAGAGTCTGATCCTGCGG
GGATTGCGGAATGGGCGGTTCCTTTGATCAGGAAAGGAAAAGCAGCGGCGATAATCGATA
GGAACATTTGTTTGCCAAGAAATGTAGAGCCTTTGCTTAAATTGGCTGAGTTAGCTGAGC
TTGCTGTGAGGGAAAATTCGAATGAACGACCAAATATCAGAAATATTCTGTGTTTTCTTG
ATCTCATCGTCAAGTCTGGCCTCACTTTTTAAGTTGCTGAAGCCTCTCAAAATGTGAAAT
ATATTCAGCATTACAAATTGACGCTTAGACAACTTAATAACACATTGCGGACGTTTTTAA
TGTACTGGGGTGGTTTTTCTTTTCACCAGTGAGACGGGCAACAGCTGATTGCCCTTCACC
GCCTGGCCCTGAGAGAGTTGCAGCAAGCGGTCCACGCTGGTTTGCCCCAGCAGGCGAAAA
TCCTGTTTGATGGTGGTTCCG
   
Sequence Name 59-K113869-SALK_007997C-R210
GenBank Accession LN485717 [GenBank]
Sequencing primer T-DNA primer, R210: 5'-CCCAGTACATTAAAAACGTCCGC-3'
Sequence (A. th genome BLAST matches underlined) 
>59-K113869-SALK_007997C-R210
GTCATTTGTATGCTGAATAATTTCACATTTTGAGAGGCTTCAGCAACTTAAAAAGTGAGG
CCAGACTTGACGATGAGATCAAGAAAACACAGAATATTTCTGATATTTGGTCGTTCATTC
GAATTTTCCCTCACAGCAAGCTCAGCTAACTCAGCCAATTTAAGCAAAGGCTCTACATTT
CTTGGCAAACAAATGTTCCTATCGATTATCGCCGCTGCTTTTCCTTTCCTGATCAAAGGA
ACCGCCCATTCCGCAATCCCCGCAGGATCAGACTCTCTATCGATAGCTTTTCGCCCGCTT
AGTATCTCAAGTAGAACAATACCAAAGTCATACACATCACCTTCTCTATCACTATTGCTT
GAATCCCTTTCATTCGAGCTTACCAATCCGAAATCCGCAATTCTTGCGCACATTTCACCG
TCTAACAAGATGTTGGACGTTTTCACATCTCTGTGAATTATCGGAGGATCTACTTCGTTG
TGTAAGTAATCAAGTCCTCTTGCAGCCTGCAACATGATCTTCAGTCTCATGCTCCAGTCC
AGTTGTGAAGATACTCC

Last Updated on 06.12.2018 16:11