Sequences from confirmation amplicon


Confirmation sequences for line SALK_008365c and hit At2g19090 are shown.
Line ID SALK_008365c
Locus At2g19090 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr2:8265388
Primers used for PCR 23um - LBb3, show primer details
   
Sequence Name 02-K117319-SALK_008365C-23um
GenBank Accession LN484318 [GenBank]
Sequencing primer gene specific primer, 23um: 5'-TGATGCCAAAAACCTACCTAATTC-3'
Sequence (A. th genome BLAST matches underlined) 
>02-K117319-SALK_008365C-23um
AATTATACAGTACTAGGAATTGAATTTACATCCGGGGGCTGCTGCTCCGACGCATCGTCT
GCTGGTTGGCTAACAAGCTTCGAGTGTTCATCGGCCGAGTTGATGGAAAACTCAGCTAGT
GAACTAACTGCAACAGACATTCCATGGCAGAGAACCTTCACAGCAACCTCTGCAAGTTTC
TCAGCAGTCATCACAACATCTTCTTCTCCCTTATCAGCCACTACAAGCTCCATGCTTTCT
GACCCGGACAACTTAATAACACATTGCGGACGTTTTTAATGTACTGGGGTGGTTTTTCTT
TTCACCAGTGAGACGGGCAACAGCTGATTGCCCTTCACCGCCTGGCCCTGAGAGAGTTGC
AGCAAGCGGTCCACGCTGGTTTGCCCCAGCAGGCGAAAATCCTGTTTGATGGTGGTTCCG
AAATGGGGCAAAAT
   
Sequence Name 18-K117318-SALK_008365C-R210
GenBank Accession LN484793 [GenBank]
Sequencing primer T-DNA primer, R210: 5'-CCCAGTACATTAAAAACGTCCGC-3'
Sequence (A. th genome BLAST matches underlined) 
>18-K117318-SALK_008365C-R210
AGTGGCTGATAAGGGAGAAGAAGATGTTGTGATGACTGCTGAGAAACTTGCAGAGGTTGC
TGTGAAGGTTCTCTGCCATGGAATGTCTGTTGCAGTTAGTTCACTAGCTGAGTTTTCCAT
CAACTCGGCCGATGAACACTCGAAGCTTGTTAGCCAACCAGCAGACGATGCGTCGGAGCA
GCAGCCCCCGGATGTAAATTTCAATTCCTAGTACTTGTTATTAATTTTGTTTGGTTTTAG
AGAGTTTAGAATTAGGTAGGTTTTTGGCATCA

Last Updated on 06.12.2018 16:11