Sequences from confirmation amplicon


Confirmation sequences for line SALK_008497c and hit At1g80440 are shown.
Line ID SALK_008497c
Locus At1g80440 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:30242151
Primers used for PCR 50ew - LBb3, show primer details
   
Sequence Name 53-K111283-SALK_008497C-50ew
GenBank Accession LN485580 [GenBank]
Sequencing primer gene specific primer, 50ew: 5'-TTAACCGTTTTTGCCCATACTAAC-3'
Sequence (A. th genome BLAST matches underlined) 
>53-K111283-SALK_008497C-50ew
CAACACAAAAATTATATTAAAAAAAGAAAGAAACGATGAATGAATCAACAAGTTTTCTAG
AAACACTTATCTTCAAATATCTATAAAGCGGCACACTCTCCTCTTTCCCATCATCATCAT
CATCTTCTTCAACCTTGTCTCGATCCAAAATTTCATCTTTTACCAAAACTATTACAATAT
CCCTCACTCTACTTGTAAAGCTTCAATCTTCTTCACTTCTCAACAAACAGATAAACAAAA
AGCTTCAATCTTTGATACTTTCTCGATGGAACTTATCCCCAATCTTCCCGACGACGTTGC
TCGCGAGTGTCTCCTTCGCTCCTCCTACCAACAGTTCCCTGTCATCGCCTCCGTTTGCAG
AGCCTGGAACCGTGAAGTCTCTCTCTCTCAGTTTTTACATCAGCGAAAAGCTTCACGCCA
TAGCCAAGAGCTTCTTATTCTGTCTCAGGCTCGTGTCGACCCCGCTGGATCCGGAAAGAT
CATCGCTACGCCTGAATACCGGATCTCTGTTCTCGAATCCGGGTCGGGTCTTTGGACGGA
GCTTCCTCCGATTCCTGGTCAAACCAAAGGACTTCCTCTGTTCTGCAGATTGGTTTCCGT
TGGATCCGATCTTATTGTCTTGGGTGGGCTTGACCCGATTACGTGGCAGGCCCATGATTC
GGTATTCGTCTTAGACAACTTAATAACACATTGCGGACGCTTTTAATGTACTGGGGTGGT
TTTTCTTTTCACCAGTGAGACGGGCAACAGCTGATTGCCCTTCACCGCCTGGCCCTGAGA
GAGTTGCAGCAAGCGGTCCACGCTGGTTTGCCCCAGCAGGCGAAAATCCTGTTTGATGGT
GGTTC
   
Sequence Name 37-K111282-SALK_008497C-R210
GenBank Accession LN485249 [GenBank]
Sequencing primer T-DNA primer, R210: 5'-CCCAGTACATTAAAAACGTCCGC-3'
Sequence (A. th genome BLAST matches underlined) 
>37-K111282-SALK_008497C-R210
GATCTGGGCCTGCCCGTAATCGGGTCAAGCCCACCCAAGACAATAAGATCGGATCCAACG
GAAACCAATCTGCAGAACAGAGGAAGTCCTTTGGTTTGACCAGGAATCGGAGGAAGCTCC
GTCCAAAGACCCGACCCGGATTCGAGAACAGAGATCCGGTATTCAGGCGTAGCGATGATC
TTTCCGGATCCAGCGGGGTCGACACGAGCCTGAGACAGAATAAGAAGCTCTTGGCTATGG
CGTGAAGCTTTTCGCTGATGTAAAAACTGAGAGAGAGAGACTTCACGGTTCCAGGCTCTG
CAAACGGAGGCGATGACAGGGAACTGTTGGTAGGAGGAGCGAAGGAGACACTCGCGAGCA
ACGTCGTCGGGAAGATTGGGGATAAGTTCCATCGAGAAAGTATCAAAGATTGAAGCTTTT
TGTTTATCTGTTTGTTGAGAAGTGAAGAAGATTGAAGCTTTACAAGTAGAGTGAGGGATA
TTGTAATAGTTTTGGTAAAAGATGAAATTTTGGATCGAGACAAGGTTGAAGAAGATGATG
ATGATGATGGGAAAGAGGAGAGTGTGCCGCTTTATAGATATTTGAAGATAAGTGTTTCTA
GAAAACTTGTTGATTCATTCATCGTTTCTTTCTTTTTTTAATATAATTTTTGTGTTGTAT
ATTTTATAGTTTAAATCAAATAAAATGAAGATAACGGAATTTGAGTTAGTATGGGCAAAA
CCGGTT

Last Updated on 06.12.2018 16:11