Sequences from confirmation amplicon


Confirmation sequences for line SALK_008606c and hit At5g67300 are shown.
Line ID SALK_008606c
Locus At5g67300 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:26854021
Primers used for PCR 51ew - LBb3, show primer details
   
Sequence Name 54-K111283-SALK_008606C-51ew
GenBank Accession LN485603 [GenBank]
Sequencing primer gene specific primer, 51ew: 5'-CTCATGTAAAGCCCAGTAACAACA-3'
Sequence (A. th genome BLAST matches underlined) 
>54-K111283-SALK_008606C-51ew
ACCGGCCGATGATCCTCCGAACCATCGTAACCCCGATGGTCGTAACCGCCGCATTTCCTC
TTGAGCGTCGAGTTCCAGTGATTCTTCACGGCGTTGTCCGTACGACCGTTGAGAAGACGA
GCAATCGTCGCCCATTTATTACCGAACTGAGCGTGAGCACGTGCGATCGTCTCGTCTTCC
TCAGCCGAAAACGGCCGATGCTCAACTTGCGGCGAAAGCTGGTTGCACCACCGTAAACGA
CACGATTTCCCCGATCTACCGGGAATAGATTTGCTAATCACTGTCCAGTTTCTTGGACCG
TATTTAACAACAAGCCTACGAAGCTGCTCGTCTTCTTCAGGACTCCATGGACCTTTGATC
CTATCAGCCATTGATTTTGGAATGTTTTATCAAACTTTAGCAATCTTCTCCTCTTTAGTG
ATTTTGGTTTTGAGAGATGAACCAAAAACAAGTTCGAGAGAGGACAAAATTATTGTGGTG
TAAACAAATTGACGCTTAGACAACTTAATAACACATTGCGGACGTTTTTAATGTACTGGG
GTGGTTTTTCTTTTCACCAGTGAGACGGGCAACAGCTGATTGCCCTTCACCGCCTGGCCC
TGAGAGAGTTGCAGCAAGCGGTCCACGCTGGTTTGCCCCAGCAGGCGAAAATCCTGTTTG
ATGGTGGTTCCG
   
Sequence Name 38-K111282-SALK_008606C-R210
GenBank Accession LN485262 [GenBank]
Sequencing primer T-DNA primer, R210: 5'-CCCAGTACATTAAAAACGTCCGC-3'
Sequence (A. th genome BLAST matches underlined) 
>38-K111282-SALK_008606C-R210
CATTTGTTTACCCACAATAATTTTGTCCTCTCTCGAACTTGTTTTTGGTTCATCTCTCAA
AACCAAAATCACTAAAGAGGAGAAGATTGCTAAAGTTTGATAAAACATTCCAAAATCAAT
GGCTGATAGGATCAAAGGTCCATGGAGTCCTGAAGAAGACGAGCAGCTTCGTAGGCTTGT
TGTTAAATACGGTCCAAGAAACTGGACAGTGATTAGCAAATCTATTCCCGGTAGATCGGG
GAAATCGTGTCGTTTACGGTGGTGCAACCAGCTTTCGCCGCAAGTTGAGCATCGGCCGTT
TTCGGCTGAGGAAGACGAGACGATCGCACGTGCTCACGCTCAGTTCGGTAATAAATGGGC
GACGATTGCTCGTCTTCTCAACGGTCGTACGGACAACGCCGTGAAGAATCACTGGAACTC
GACGCTCAAGAGGAAATGCGGCGGTTACGACCATCGGGGTTACGATGGTTCGGAGGATCA
TCGGCCGGTTAAGAGATCGGTGAGTGCGGGATCTCCACCTGTTGTTACTGGGCTTTTACA
TGA

Last Updated on 06.12.2018 16:11