Sequences from confirmation amplicon


Confirmation sequences for line SALK_009272 and hit At3g50400 are shown.
Line ID SALK_009272
Locus At3g50400 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:18706143
Primers used for PCR 49qz - LBb3, show primer details
   
Sequence Name 10-K115992-SALK_009272C-49qz
GenBank Accession LN484560 [GenBank]
Sequencing primer gene specific primer, 49qz: 5'-CGGTTAGGAATGGACATACAAGTT-3'
Sequence (A. th genome BLAST matches underlined) 
>10-K115992-SALK_009272C-49qz
AGTTTGATAGCTTTTGGGTCAAGATAAAGCTAGAGATTACATTAGAAAGAGATCCTTATT
TTCTGTAGTTATCGGTTCAAACGATTTTCTCAACAATTACCTAGTCCCTTTTGTCGCGGC
TCAAGCAAGACTAACACAAACTCCTGAAACTTTCGTCGATGACATGATTAGCCACCTCCG
CAACCAGCTCAAGGTATGTTATAAAACATATATAATATTATTTCCGTTTAACGTTAATTG
AAGGATTTTTTTTAATCAAATGTAGAGATTATATGATATGGACGCTCGGAAATTTGTGGT
GGGAAATGTGGCTCCTATTGGATGTATACCATACCAGAAATCGATCAATCAATTGAACGA
TAAACAGTGTGTTGATTTAGCAAACAAACTGGCTATTCAATACAATGCTCGTCTCAAAGA
TCTCTTGACGGTGGAATTGAAAGATAGTCTCAAAGACGCACACTTCGTTTATGCCAACGT
TTATGATTTATTCATGGACCTCATTGTCAACTTCAAAGACTACGGTAATAATATATACTA
AAGGCCCATGGGCCTTTTAAAAACTCTTATCTTCAGGATATATTGTGGTGTAAACAAATT
GACGCTTAGACAACTTAATAACACATTGCGGACGTTTTTAATGTACTGGGGTGGTTTTTC
TTTTCACCAGTGAGACGGGCAACAGCTGATTGCCCTTCACCGCCTGGCCCTGAGAGAGTT
GCAGCAAGCGGTCCACGCTGGTTTGCCCCAGCAGGCGAAAATCCTGTTTGATGGTGGTTC
C
   
Sequence Name 01-K115991-SALK_009272C-R210
GenBank Accession queueing for submission to ENA/GenBank
Sequencing primer T-DNA primer, R210: 5'-CCCAGTACATTAAAAACGTCCGC-3'
Sequence (A. th genome BLAST matches underlined) 
>01-K115991-SALK_009272C-R210
CAAGAGGAGCCACATGGCGCAACAAAAAGCGCCGAGCGCACGAATAACACAAGACCAACA
GAGGAACAAAAAAAACCCTAAAAGGCGCGGAGAACGGAAAAAAAAACAGGCAGGTCAAAA
AACAGACCGTGGGCCGGAGGCGGAGGCGGCGAAACC

Last Updated on 06.12.2018 16:11