Sequences from confirmation amplicon


Confirmation sequences for line SALK_009348c and hit At3g51870 are shown.
Line ID SALK_009348c
Locus At3g51870 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:19244910
Primers used for PCR 05tw - LBb3, show primer details
   
Sequence Name 13-K116922-SALK_009348C-05tw
GenBank Accession LN484650 [GenBank]
Sequencing primer gene specific primer, 05tw: 5'-GCAGACACATGGTATAAGACTTGG-3'
Sequence (A. th genome BLAST matches underlined) 
>13-K116922-SALK_009348C-05tw
TAGTTTGTTTTTGTTGATGTTCGTGTGTGTATGTACGTTAGTCTTTCATGTTATAAATGA
TGCAAGAGCTTACTGGAAATGATTTTGTAGGCAATCACTTTGATAGCTAAAGAAGAAGGA
GTTAAAGGTTATTGGAAAGGAAATTTACCTCAGGTAATTGTTTGGACTTGTTTTTTTTAT
ATCAAGTGATTGGTTTTGTCTTGACTTATTGGATAATTCTGTTTGAAAATGTGACATCAG
GTGATAAGAGTGTTACCTTACAGTGCGGTCCAGCTTTTGGCTTATGAGAGTTACAAGGTG
AGTTTGTGTCATCTATAGCTTATTGGCTATTGGGATTATGTACTAAAGAGAAATAGAAAT
GATTGTTATAAAATTCTGTAGCTACTTGTCCTAGAAAACAAATTAAACAAATTGACGCTT
AGACAACTTAATAACACATTGCGGACGTTTTTAATGTACTGGGGTGGTTTTTCTTTTCAC
CAGTGAGACGGGCAACAGCTGATTGCCCTTCACCGCCTGGCCCTGAGAGAGTTGCAGCAA
GCGGTCCACGCTGGTTTGCCCCAGCAGGCGAAAATCCTGTTTGATGGTGGTTCCG
   
Sequence Name 02-K116921-SALK_009348C-R210
GenBank Accession LN484316 [GenBank]
Sequencing primer T-DNA primer, R210: 5'-CCCAGTACATTAAAAACGTCCGC-3'
Sequence (A. th genome BLAST matches underlined) 
>02-K116921-SALK_009348C-R210
TATTTGTTTTCTAGGACAAGTAGCTACAGAATTTTATAACAATCATTTCTATTTCTCTTT
AGTACATAATCCCAATAGCCAATAAGCTATAGATGACACAAACTCACCTTGTAACTCTCA
TAAGCCAAAAGCTGGACCGCACTGTAAGGTAACACTCTTATCACCTGATGTCACATTTTC
AAACAGAATTATCCAATAAGTCAAGACAAAACCAATCACTTGATATAAAAAAAACAAGTC
CAAACAATTACCTGAGGTAAATTTCCTTTCCAATAACCTTTAACTCCTTCTTCTTTAGCT
ATCAAAGTGATTGCCTACAAAATCATTTCCAGTAAGCTCTTGCATCATTTATAACATGAA
AGACTAACGTACATACACACACGAACATCAACAAAAACAAACTTACCTCGATAAATCCTA
TCGCCTTCTTCGCACTCTGTTGTCCAAGTCTTA

Last Updated on 06.12.2018 16:11