Sequences from confirmation amplicon


Confirmation sequences for line SALK_009879c and hit At1g19220 are shown.
Line ID SALK_009879c
Locus At1g19220 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:6631977
Primers used for PCR 45us - LBb3, show primer details
   
Sequence Name 15-K117668-SALK_009879C-R210
GenBank Accession LN484707 [GenBank]
Sequencing primer T-DNA primer, R210: 5'-CCCAGTACATTAAAAACGTCCGC-3'
Sequence (A. th genome BLAST matches underlined) 
>15-K117668-SALK_009879C-R210
CATTTGTTTACCCACAATTTGTTTAGCTTTAACCAAGATAGCACAAGAAGAACGATATAA
TTCAGACCTTATTCACAGGTTGAAGAGTCATTTGTGCATAGACTTCATCTGTTTCGGTAT
CAGCCTGATTCAGAATTTCACAAAAGTCCTGATTAGTTCATCTTCTTGTTGATTCACAAG
TTGGAGTCAGAAGAGACTTACATGTAATGTAACACTGTGAAGCAAGCAAATCAGCTTAGA
AGGAAGATTTGGGTAATTTGGTATAAAATCTGTTTGCTTCTGCATCGATGCTGCAACCTG
AAAAAAGGTTTCATTGAACATATTACTTAGATTGCTACCAAAGGTTTCAAAATAGTTTGA
AACGCAATGAACTTACTTGCTCGCTGTGTCCTTGAGGGAAGTAAACCACAAGACTTCCCA
CAGGAGGTAATGAAACTAAAGGCCCTGCACAAGCGTGCCATAGTTGAGAATTGATTGGCT
TCTTCTCTCCTGTTCTCCATCATCACA
   
Sequence Name 52-K117669-SALK_009879C-45us
GenBank Accession LN485565 [GenBank]
Sequencing primer gene specific primer, 45us: 5'-GTGATGATGATGAACAGGAGAGAA-3'
Sequence (A. th genome BLAST matches underlined) 
>52-K117669-SALK_009879C-45us
GGCCTTTAGTTTCATTACCTCCTGTGGGAAGTCTTGTGGTTTACTTCCCTCAAGGACACA
GCGAGCAAGTAAGTTCATTGCGTTTCAAACTATTTTGAAACCTTTGGTAGCAATCTAAGT
AATATGTTCAATGAAACCTTTTTTCAGGTTGCAGCATCGATGCAGAAGCAAACAGATTTT
ATACCAAATTACCCAAATCTTCCTTCTAAGCTGATTTGCTTGCTTCACAGTGTTACATTA
CATGTAAGTCTCTTCTGACTCCAACTTGTGAATCAACAAGAAGATGAACTAATCAGGACT
TTTGTGAAATTCTGAATCAGGCTGATACCGAAACAGATGAAGTCTATGCACAAATGACTC
TTCAACCTGTGAATAAGGTCTGAATTATATCATTCTTCTTGTGCTATCTTGGTTTAAAGC
TAAACAAATTGTGGTGTAAACAAATTGACGCTTAGACAACTTAATAACACATTGCGGACG
TTTTTAATGTACTGGGGTGGTTTTTCTTTTCACCAGTGAGACGGGCAACAGCTGATTGCC
CTTCACCGCCTGGCCCTGAGAGAGTTGCAGCAAGCGGTCCACGCTGGTTTGCCCCAGCAG
GCGAAAATCCTGTTTGATGGTGGTTCCG

Last Updated on 06.12.2018 16:11