Sequences from confirmation amplicon


Confirmation sequences for line SALK_010185 and hit At1g21780 are shown.
Line ID SALK_010185
Locus At1g21780 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:7654011
Primers used for PCR 50qz - LBb3, show primer details
   
Sequence Name 11-K115992-SALK_010185C-50qz
GenBank Accession LN484591 [GenBank]
Sequencing primer gene specific primer, 50qz: 5'-CCTCTCAGCTAGCTCAACTGTTTT-3'
Sequence (A. th genome BLAST matches underlined) 
>11-K115992-SALK_010185C-50qz
GAAGGATCATCCACATCCCATAGACGACATGTCGAGAGAATCTTGCATGGCTCTACTAAG
TTACCTCTACGGAAACATAACCCAAGAAGAGTTCTGGAAACACAGGCTCGCCCTCCTCGG
CGCTGCAAACAAGTACGACATAACGGATTTGAAAGCAGCCTGCGAGGAGAGCCTAATGGA
AGATATAAACTCGAGTAATGTGCTCGAGAGGCTACAAGAAGCTTGGCTTTATCAGCTGGA
GAAACTGAAGAAAGGGTGTTTGATGTATTTGTTTGATTTCGGAAAGATTTATGATGTCAG
AGAAGAAATCAGCAGTTTCTTCAGGCAAGCTGATCGAGAACTGATGCTGGAGATGTTTCA
AGAGGTTTTGTCTGTGTGGAAACCGGTCTAATATACACTTACACACATACTTTGAACATT
TCAGCTTGATGGTGTTTTGTTTATAGTCTTCTTGTTCTCTTCTGTGTGTGTAACTAACAA
CAACTCTGGATTTTGTTGTATCACTCTGTCTAATGTATAGTATTGAGTGGATTTGCGGAC
AAATTGTGGACAACAGCTGACAACTTATTAATGTACCGACCGGCGGGAAACTTAATAGAT
AGAATGGACTAACTGCACCACAATATATTGTGGTGTAAACAAATTGACGCTTAGACAACT
TAATAACACATTGCGGACGTTTTTAATGTACTGGGGTGGTTTTTCTTTTCACCAGTGAGA
CGGGCAACAGCTGATTGCCCTTCACCGCCTGGCCCTGAGAGAGTTGCAGCAAGCGGTCCA
CGCTGGTTTGCCCCAGCAGGCGAAAATCCTGTTTGATGGTGGTTC
   
Sequence Name 02-K115991-SALK_010185C-R210
GenBank Accession queueing for submission to ENA/GenBank
Sequencing primer T-DNA primer, R210: 5'-CCCAGTACATTAAAAACGTCCGC-3'
Sequence (A. th genome BLAST matches underlined) 
>02-K115991-SALK_010185C-R210
AAGCTCATCAAGAGCTTTTCTGTCTTCCACCAGGCTCTCCCCGCAAGTTGTGTTCAAATC
TGTTATGTTCTACTTGTTCGCAACAAAAAAGACGGCGAGCTTGT

Last Updated on 06.12.2018 16:11