Sequences from confirmation amplicon


Confirmation sequences for line SALK_010501c and hit At3g51730 are shown.
Line ID SALK_010501c
Locus At3g51730 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:19188354
Primers used for PCR 05rb - LBb3, show primer details
   
Sequence Name 63-K116104-SALK_010501C-05rb
GenBank Accession LN485828 [GenBank]
Sequencing primer gene specific primer, 05rb: 5'-TTTCGACCGTTTTTAATCATTCAT-3'
Sequence (A. th genome BLAST matches underlined) 
>63-K116104-SALK_010501C-05rb
GGGCTCAAATATTATTTCATGGAATTCCTAATCTTTATATGACTTGGTCAAATCTTGACA
CTTTTGGCCACGTCAGTCCTAGTACCAATCGTGCGGCACACTAGATATATTCGTGGTAAT
AATATGTAATTTTTTTTGAGAAATCCTTCAAACGTGTGGCAAACTACACTAATATCATTC
ATATAAAATGTAACAAACCTCAAATAGAATACAGTGATATCATAGTAGAGGTACAATTGT
TGTAGCGATCATTATTTACACAAAGGCAAAGGGACATTTTGGAGGATGTGTTTGAATCCA
TACATATAGACACTACACAAAGTCTGCAACCATTCTCATGGAGCAGCTCGTGTTCCTACT
TGTATAACCCATAAGGAATTAAAATCCTGTTATAGCAACGTTTTGAACATTCAAGAATCA
GCCAACTCCGGCTGCCTTAGAACCGATTTCTCAGGGGGGCATGCGCGCAAGAGTGTGCAG
ACGTCGTTTTTCAGAAAATATCAGTTTAAACTATAAGCGTCAGCGGTCGTTTTTCAGGAT
ATATTGTGGTGTAAACAAATTGACGCTTAGACAACTTAATAACACATTGCGGACGTTTTT
AATGTACTGGGGTGGTTTTTCTTTTCACCAGTGAGACGGGCAACAGCTGATTGCCCTTCA
CCGCCTGGCCCTGAGAGAGTTGCAGCAAGCGGTCCACGCTGGTTTGCCCCAGCAGGCGAA
AATCCTGTTTGATGGTGGTTCC
   
Sequence Name 58-K116103-SALK_010501C-R210
GenBank Accession LN485697 [GenBank]
Sequencing primer T-DNA primer, R210: 5'-CCCAGTACATTAAAAACGTCCGC-3'
Sequence (A. th genome BLAST matches underlined) 
>58-K116103-SALK_010501C-R210
TCCCACAATATATCCTGAAAACGACCGCTGACGCTTATAGTTCCATTGATATTTTCTGAA
AAACGACGTCTGCACACTCTTGCGCGCATGCCCCCCTGAGAAATCGGTTCTAAGGCAGCC
GGAGTTGGCTGATTCTTGAATGTTCAAAACGTTGCTATAACAGGATTTTAATTCCTTATG
GGTTATACAAGTAGGAACACGAGCTGCTCCATGAGAATGGTTGCAGACTTTGTGTAGTGT
CTATATGTATGGATTCAAACACATCCTCCAAAATGTCCCTTTGCCTTTGTGTAAATAATG
ATCGCTACAACAATTGTACCTCTACTATGATATCACTGTATTCTATTTGAGGTTTGTTAC
ATTTTATATGAATGATATTAGTGTAGTTTGCCACACGTTTGAAGGATTTCTCAAAAAAAA
TTACATATTATTACCACGAATATATCTAGTGTGCCGCACGATTGGTACTAGGACTGACGT
GGCCAAAAGTGTCAAGATTTGACCAAGTCATATAAAGATTAGTGAATTCCATTGAAATAA
TTATTTTGATGCCCCAACGAGTCTCGAGACATTTTTAGTAATAATGAATGATTAAAAACG
GTCGAAAAGGTTC

Last Updated on 06.12.2018 16:11