Sequences from confirmation amplicon


Confirmation sequences for line SALK_010839c and hit At3g04120 are shown.
Line ID SALK_010839c
Locus At3g04120 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:1082981
Primers used for PCR 10bb - LBb3, show primer details
   
Sequence Name 22-K108069-SALK_010839C-10bb
GenBank Accession LN484905 [GenBank]
Sequencing primer gene specific primer, 10bb: 5'-GATTTGGAATTGTTGAGGGTCTTA-3'
Sequence (A. th genome BLAST matches underlined) 
>22-K108069-SALK_010839C-10bb
TATTTTCATCAGTTAGAAGTTTATTACAAACTTGCTTGCCTATAGGTGGAAAATTTGTGA
TTTAATGGGGTTTGCTTTATGATTTCAGCTACTCAGAAGACTGTTGATGGGCCTTCAATG
AAGGACTGGAGAGGTGGAAGAGCTGCTTCATTCAACATTATTCCCAGCAGCACTGGAGCT
GCCAAGGCTGTCGGAAAGGTGCTTCCAGCTCTTAACGGAAAGTTGACTGGAATGTCTTTC
CGTGTCCCAACCGTTGATGTCTCAGTTGTTGACCTTACTGTCAGACTCGAGAAAGCTGCT
ACCTACGATGAAATCAAAAAGGCTATCAAGTAAGCTTTTGAGCAATGACAGATTAAGTTT
ACTTATATTCCAGTAGTGATCAAATTACTCACCAAGTGTTTTTACCACCAATACATAGGG
AGGAATCCGAAGGCAAACTCAAGGGAATCCTTGGATACACCGAGGATGATGTTGTCTCAA
ATTGACGCTTAGACAACTTAATAACACATTGCGGACGTTTTTAATGTACTGGGGTGGTTT
TTCTTTTCACCAGTGAGACGGGCAACAGCTGATTGCCCTTCACCGCCTGGCCCTGAGAGA
GTTGCAGCAAGCGGTCCACGCTGGTTTGCCCCAGCAGGCGAAAATCCTGTTTGATGGTGG
TTCCGA
   
Sequence Name 33-K108070-SALK_010839C-R210
GenBank Accession LN485181 [GenBank]
Sequencing primer T-DNA primer, R210: 5'-CCCAGTACATTAAAAACGTCCGC-3'
Sequence (A. th genome BLAST matches underlined) 
>33-K108070-SALK_010839C-R210
GCGTCATTTGAGACACATCATCCTCGGTGTATCCAGGATTCCCTTGAGTTTGCCTTCGGA
TTCCTCCCTATGTATTGGTGGTAAAAACACTTGGTGAGTAATTTGATCACTACTGGAATA
TAAGTAAACTTAATCTGTCATTGCTCAAAAGCTTACTTGATAGCCTTTTTGATTTCATCG
TAGGTAGCAGCTTTCTCGAGTCTGACAGTAAGGTCAACAACTGAGACATCAACGGTTGGG
ACACGGAAAGACATTCCAGTCAACTTTCCGTTAAGAGCTGGAAGCACCTTTCCGACAGCC
TTGGCAGCTCCAGTGCTGCTGGGAATAATGTTGAATGAAGCAGCTCTTCCACCTCTCCAG
TCCTTCATTGAAGGCCCATCAACAGTCTTCTGAGTAGCTGAAATCATAAAGCAAACCCCA
TTAAATCACAAATTTTCCACCTATAGGCAAGCAAGTTTGTAATAAACTTCTAACTGATTG
ATAAATTTACCAGTGATTGAGTGGACTGTAGTCATAAGACCCTCAACAANTCCAAAT

Last Updated on 06.12.2018 16:11