Sequences from confirmation amplicon


Confirmation sequences for line SALK_011390 and hit At2g16250 are shown.
Line ID SALK_011390
Locus At2g16250 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr2:7042166
Primers used for PCR 01as - R204, show primer details
   
Sequence Name 08-K107313-SALK_011390C-01as
GenBank Accession LN484480 [GenBank]
Sequencing primer gene specific primer, 01as: 5'-ATGCATAGAACCAGAATAATTGGC-3'
Sequence (A. th genome BLAST matches underlined) 
>08-K107313-SALK_011390C-01as
TGCTGCCATATAATTACTGTTCTGCGGCTTATCCCTGAAGAAGCATTTTTCGATGTCGGT
TGTGTCAAATTAGGACGTCCGAAATCATCAAAATCTAATCCTCTAGACTTATAGAATGCT
GCACATATTGCTGACGGCTTCTGTCTCCTCTCATTTCGAAGGCAATTGCTTGTAACAGAC
ACATTTTCCCCGGTCACATAATCAGGAAGCTTACCTTCGAAATAATTACCAGAGAGGTCC
ATTATCCTGAACCGTCTAAGAATCGGTGTGAGCTCTCCATAGAATGTGTTCGAAGAGATA
TCAACCATTTCAGCTATCTGGTCTGAATCATAACTAGAATATGGCAGCAGTCCTGTGAAG
TTGTTCTTAGCAATGTCAAGAATCCGCAATTTTGGCAACGACCAGCACACATCAGGTAGA
CTACCAGAGAAACCATTTTCCCTCAAAACTAAAGTTTGCAGTTGGCTCTCAGCACTAAAC
AAATCTACTGGTAAAGTACCACTCAAAAGGTTATCGCCAATCGCCATTAGCTGCAACTTA
CTTAACTTTCTTAGCTCCTGAGGAACTGAACCAGACAATGAATTGATGTACAAATTGACA
ACTTGACAAATTAATAACACATTGTGGTGTTTACAAATTGACGCTTAGACAACTTAATAA
CACATTGCGGACGTTTTTAATGTACTGGGGTGGTTTTTCTTTTCACCAGTGAGACGGGCA
ACAGCTGATTGCCCTTCACCGCCTGGCCCTGAGAGAGTTGCAGCAAGC
   
Sequence Name 48-K107289-SALK_011390C-R210
GenBank Accession LN485451 [GenBank]
Sequencing primer T-DNA primer, R210: 5'-CCCAGTACATTAAAAACGTCCGC-3'
Sequence (A. th genome BLAST matches underlined) 
>48-K107289-SALK_011390C-R210
GTGCTAGCGTCATTTGTAACCCACAATGTGTTATTAATTTGTCAAGTTGTCAATTTGTAC
ATCAATTCATTGTCTGGTTCAGTTCCTCAGGAGCTAAGAAAGTTAAGTAAGTTGCAGCTA
ATGGCGATTGGCGATAACCTTTTGAGTGGTACTTTACCAGTAGATTTGTTTAGTGCTGAG
AGCCAACTGCAAACTTTAGTTTTGAGGGAAAATGGTTTCTCTGGTAGTCTACCTGATGTG
TGCTGGTCGTTGCCAAAATTGCGGATTCTTGACATTGCTAAGAACAACTTCACAGGACTG
CTGCCATATTCTAGTTATGATTCAGACCAGATAGCTGAAATGGTTGATATCTCTTCGAAC
ACATTCTATGGAGAGCTCACACCGATTCTTAGACGGTTCAGGATAATGGACCTCTCTGGT
AATTATTTCGAAGGTAAGCTTCCTGATTATGTGACCGGGGAAAATGTGTCTGTTACAAGC
AATTGCCTTCGAAATGAGAGGAGACAGAAGCCGTCAGCAATATGTGCAGCATTCTATAAG
TCTAGAGGATTAGATTTTGATGATTTCGGACGTCCTAATTTGACACAACCGACATCGAAA
AATGCTTCTTCAGGGATAAGCCGCAGAACAGTAATTATATTGGCAGCAGTGGGTGGTGGA
GTTGCTTTTATTCTTCTTTTTGTTATCTTGCCAATTATTCTGGTCCTATGCA

Last Updated on 06.12.2018 16:11