Sequences from confirmation amplicon


Confirmation sequences for line SALK_011535c and hit At5g09760 are shown.
Line ID SALK_011535c
Locus At5g09760 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:3032716
Primers used for PCR 30gz - LBb3, show primer details
   
Sequence Name 39-K112623-SALK_011535C-30gz
GenBank Accession LN485278 [GenBank]
Sequencing primer gene specific primer, 30gz: 5'-AAAACCCAAATTAAGCCCTGTACT-3'
Sequence (A. th genome BLAST matches underlined) 
>39-K112623-SALK_011535C-30gz
TTTCCAAACCCGTCACGTTCCGTTGCTGGGTAAGTCCACGACGCGACGTTGTCTCCGAAG
TTATCGTACGATACCATCATGCTAAGCGCGTTGCTGGTGACGTGGATAAGTCCGTCGAGA
AACGACATCGTTTCACCTACTTGGCTCGTGTCGTTGACGTACTTGAGCGCTGACCATGAA
TCGTACTGGTACACTAGAGCAGCGCTCATCCAAGCTCGAGCGTCTTTGATTTTGCCACGT
GTTAAAGCTTGATCCGTCGATTGAGTGCGGTGCTCGGAGTAAGTGAGAAGCTGGAGACAC
GTGTTCGCGGCGTTTGTACGGTTTAGATTCCCTACGGACGAGTCTACAATAGACTTGATT
TTTGATTGGGCGGTAAACAAAAATAACACATTATTTAAAAGGATATATTGTGGTGTAAAC
AAATTGACGCTTAGACAACTTAATAACACATTGCGGACGTTTTTAATGTACTGGGGTGGT
TTTTCTTTTCACCAGTGAGACGGGCAACAGCTGATTGCCCTTCACCGCCTGGCCCTGAGA
GAGTTGCAGCAAGCGGTCCACGCTGGTTTGCCCCAGCAGGCGAAAATCCTGTTTGATGGT
GGTTCCGA
   
Sequence Name 15-K112622-SALK_011535C-R210
GenBank Accession LN484697 [GenBank]
Sequencing primer T-DNA primer, R210: 5'-CCCAGTACATTAAAAACGTCCGC-3'
Sequence (A. th genome BLAST matches underlined) 
>15-K112622-SALK_011535C-R210
ATTTGTTACCCACAATATATCCTTTTAATAATGTGTTATTTTTGTTTACCGCCCAATCAA
AAATCAAGTCTATTGTAGACTCGTCCGTAGGGAATCTAAACCGTACAAACGCCGCGAACA
CGTGTCTCCAGCTTCTCACTTACTCCGAGCACCGCACTCAATCGACGGATCAAGCTTTAA
CACGTGGCAAAATCAAAGACGCTCGAGCTTGGATGAGCGCTGCTCTAGTGTACCAGTACG
ATTCATGGTCAGCGCTCAAGTACGTCAACGACACGAGCCAAGTAGGTGAAACGATGTCGT
TTCTCGACGGACTTATCCACGTCACCAGCAACGCGCTTAGCATGATGGTATCGTACGATA
ACTTCGGAGACAACGTCGCGTCGTGGACTTACCCAGCAACGGAACGTGACGGGTTTTGGG
AAAAGACTGGGCCTGGTTTGGGCTTAGATCCGAGTACAGGGCTTATTNTGGGTTT

Last Updated on 06.12.2018 16:11