Sequences from confirmation amplicon


Confirmation sequences for line SALK_012128c and hit At3g23070 are shown.
Line ID SALK_012128c
Locus At3g23070 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:8203416
Primers used for PCR 18cr - R212, show primer details
   
Sequence Name 19-K108444-SALK_012128C-18cr
GenBank Accession LN484806 [GenBank]
Sequencing primer gene specific primer, 18cr: 5'-GTGGAGATATGGTTAAGGGATGAG-3'
Sequence (A. th genome BLAST matches underlined) 
>19-K108444-SALK_012128C-18cr
GTGGATTGGTGGGTCCAACAACTCCATTGCCTGAGCGACAAGCGCGATGGTCGGAAGCTC
CGCTGCTGAATTCATCATGGATCTCTATTACAGCTTCAAATCTGACGCCTCTGACTCTGG
CTCTGCCTCTGAATAATTCTTCTCAGAACTTCAAACTTTACTCTTCTTCTAAACTCATTT
CTTGCAGAATAAGTTTCTTTTGAAAGACTTCTAGTTTTAGCATCTTTCTTTATTTAATAA
TTGCTGAGACCTAAATATGGAAAACTCTTTATTTATTTGATTTGCTTGCTTTTTGAGGAC
TCAAACGGTATCGTTTTGTACTAATTAAATAGAAGCTCATTTTAGCTAGCTTGAGTTATA
TTTGATAAGATGCGCCGGTTTTGTGCTCTGTTTGGTTTAAGGTAGGGTTAACGAAAGATC
TGTATCCAAACAGTCTGATAGTTTAAACTGAAGGCGGGAAACGACAATCTGATCATGAGC
GGAGAATTAAGGGAGTCACGTTATGACCCCCGCCGATGACGCGGGACAAGCCGTTTTACG
TTTGGAACTGACAGAACCGCAACGTTGAAGGAGCCACTCAGCCGCGGGTTTCTGGAGTTT
AATGAGCTAAGCACATACTTCAGAAACCATTATTGCGCGTTCAAAAGTC
   
Sequence Name 03-K108443-SALK_012128C-R214
GenBank Accession LN484327 [GenBank]
Sequencing primer gene specific primer, R214: 5'-AACGTTGCGGTTCTGTCAGTTCC-3'
Sequence (A. th genome BLAST matches underlined) 
>03-K108443-SALK_012128C-R214
GCGGGGGTCATAACGTGACTCCCTTAATTCTCCGCTCATGATCAGATTGTCGTTTCCCGC
CTTCAGTTTAAACTATCAGACTGTTTGGATACAGATCTTTCGTTAACCCTACCTTAAACC
AAACAGAGCACAAAACCGGCGCATCTTATCAAATATAACTCAAGCTAGCTAAAATGAGCT
TCTATTTAATTAGTACAAAACGATACCGTTTGAGTCCTCAAAAAGCAAGCAAATCAAATA
AATAAAGAGTTTTCCATATTTAGGTCTCAGCAATTATTAAATAAAGAAAGATGCTAAAAC
TAGAAGTCTTTCAAAAGAAACTTATTCTGCAAGAAATGAGTTTAGAAGAAGAGTAAAGTT
TGAAGTTCTGAGAAGAATTATTCAGAGGCAGAGCCAGAGTCAGAGGCGTCAGATTTGAAG
CTGTAATAGAGATCCATGATGAATTCAGCAGCGGAGCTTCCGACCATCGCGCTTTGTCGC
TCAGGCAATGGAGTTGTTTGGACCCACCAATCCACCAAGTTATGTAGTGTCAGTGACGAA
CACACTGGCTCATCCCTTAACCATATCTCCA

Last Updated on 06.12.2018 16:11