Sequences from confirmation amplicon


Confirmation sequences for line SALK_012134c and hit At1g07750 are shown.
Line ID SALK_012134c
Locus At1g07750 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:2404967
Primers used for PCR 70by - LBb3, show primer details
   
Sequence Name 27-K108287-SALK_012134C-70by
GenBank Accession LN485054 [GenBank]
Sequencing primer gene specific primer, 70by: 5'-AACCTTGGAACAATGAAGAGAGAA-3'
Sequence (A. th genome BLAST matches underlined) 
>27-K108287-SALK_012134C-70by
GACTCTTTTCCATCCCACCAACCACCTGGACTCTTCCACTTCCACCCACAATGTAAGTCA
TCTGAAGAGCAGAGTCACAAGAGAAACCAGGCGAACACATGGAGTGTGCATCGATCCGAA
CAAGATCAGCTCCAAAGCCAACCTCTCCAACCAAAGGAAGGTTCTTGGTGTTCAAGACAA
CGACCCTGCCTCCATCCTTGATGTCTACATCAAGAGGAGCCTCCAAACAGTTCAAGACAA
ACCCAGCACGGTTCTCCTCCTTGGGCTGTGGCATCTTAAAGCCCGCATCAAGCTTCACAA
TGCCATTACCGGTTTGAGAACCAACAAGTTAATAACACATTGCGGACGTTTTTAATGTAC
TGGGGTGGTTTTTCTTTTCACCAGTGAGACGGGCAACAGCTGATTGCCCTTCACCGCCTG
GCCCTGAGAGAGTTGCAGCAAGCGGTCCACGCTGGTTTGCCCCAGCAGGCGAAAATCCTG
TTTGATGGTGGTTCCGAAATCGGCAAAA
   
Sequence Name 11-K108286-SALK_012134C-R210
GenBank Accession LN484574 [GenBank]
Sequencing primer T-DNA primer, R210: 5'-CCCAGTACATTAAAAACGTCCGC-3'
Sequence (A. th genome BLAST matches underlined) 
>11-K108286-SALK_012134C-R210
CGGTATGGCATTGTGAAGCTTGATGCGGGCTTTAGATGCCACAGCCCAAGGAGTAGAACC
GTGCTGGGTTTGTCTTGAACTGTTTGGAGGCTCCTCTTGATGTAGACATCAAGGATGGAG
GCAGGGTCGTTGTCTTGAACACCAAGAACCTTCCTTTGGTTGGAGAGGTTGGCTTTGGAG
CTGATCTTGTTCGGATCGATGCACACTCCATGTGTTCGCCTGGTTTCTCTTGTGACTCTG
CTCTTCAGGTGACTTACATTGTGGGTGGAAGTGGAAGAGTCCAGGTGGTTGGTGGTGATG
GGAAAAGAGTTCTTGAGACCCATATCAAAGCTGGTTCTCTCTTCATTGTTCCAAGGT

Last Updated on 06.12.2018 16:11