Sequences from confirmation amplicon


Confirmation sequences for line SALK_012144c and hit At1g02980 are shown.
Line ID SALK_012144c
Locus At1g02980 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:680649
Primers used for PCR 71by - LBb3, show primer details
   
Sequence Name 28-K108287-SALK_012144C-71by
GenBank Accession LN485078 [GenBank]
Sequencing primer gene specific primer, 71by: 5'-TACCTTGAACATTTTGCTGAGATG-3'
Sequence (A. th genome BLAST matches underlined) 
>28-K108287-SALK_012144C-71by
CCCACACTTTTCACTCTTCATGATCCGAACAAGAGCTGCATCAATTGCATAGCGTCTATC
TTTATCAACATCTTCAACAATTTTCTTCCTCTCATCCATCGGAGGCAGAGGCACCTGTTC
AAGAAAAACACATCATTTTTGATCCGGGCTTACATGAAAATAACAACACAACGCTAATGA
AAATATCATCATACCCTAATTCTCCTCATCTTATCGGTGAACTTGGAGTTGAATTCAAAG
GTATCGGTGTTGGAGATGTTTCTCGACATTGGTTCCTTTATAAGAATCTTGTACTTTAAG
CATGATAGTGAATGAAGCAGCCTAGACAAATTATGGTGACGAGAAATTGACAACTTAGAC
AACTTATAAATTGTCAGGATATATTGTGGTGTAAACAAATTGACGCTTAGACAACTTAAT
AACACATTGCGGACGTTTTTAATGTACTGGGGTGGTTTTTCTTTTCACCAGTGAGACGGG
CAACAGCTGATTGCCCTTCACCGCCTGGCCCTGAGAGAGTTGCAGCAAGCGGTCCACGCT
GGTTTGCCCCAGCAGGCGAAAATCCTGTTTGATGGTGGTTCCGAATCGNGCAAAA
   
Sequence Name 12-K108286-SALK_012144C-R210
GenBank Accession LN484603 [GenBank]
Sequencing primer T-DNA primer, R210: 5'-CCCAGTACATTAAAAACGTCCGC-3'
Sequence (A. th genome BLAST matches underlined) 
>12-K108286-SALK_012144C-R210
CGTCATTTGTTACCCACAATATATCCTGACAATTTATAAGTTGTCTAAGTTGTCAACTTT
TCGTCACCATAATTTGTCTAGGCTGCTTCATTCACTATCATGCTTAAAGTACAAGATTCT
TATAAAGGAACCAATGTCGAGAAACATCTCCAACACCGATACCTTTGAATTCAACTCCAA
GTTCACCGATAAGATGAGGAGAATTAGGGTATGATGATATTTTCATTAGCGTTGTGTTGT
TATTTTCATGTAAGCCCGGATCAAAAATGATGTGTTTTTCTTGAACAGGTGCCTCTGCCT
CCGATGGATGAGAGGAAGAAAATTGTTGAAGATGTTGATAAAGATAGACGCTATGCAATT
GATGCAGCTCTTGTTCGGATCATGAAGAGTAGAAAAGTGTTGGGGCATCAACAGTTGGTC
TCTGAATGTGTTGAGCATCTCAGCAAAATGTTCAAGGTA

Last Updated on 06.12.2018 16:11