Sequences from confirmation amplicon


Confirmation sequences for line SALK_012199 and hit At4g28360 are shown.
Line ID SALK_012199
Locus At4g28360 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr4:14029963
Primers used for PCR 07rb - LBb3, show primer details
   
Sequence Name 65-K116055-SALK_012199C-07rb
GenBank Accession LN485880 [GenBank]
Sequencing primer gene specific primer, 07rb: 5'-ATTGATATAATCCCGCTTCTTCCT-3'
Sequence (A. th genome BLAST matches underlined) 
>65-K116055-SALK_012199C-07rb
CTTCAACGTCCCTTCCCACAAATGCTTCCGCTGCAATCCATATCACAAGCTTAGATGAAA
TACATCGTTCTGGCATATCATGTTTGCTTCCTTAAAGTACCAATCGGCGAACTCTCTAAA
ATATCACGTTCTCTTTTTAAATGTAAAACAAGGAAATCAGGGATCCAGAATCAGTTTTAC
ATACCAACAAGGAGACGGTCAGGATCTAGTCCATGGTTATGAGTAGCATTTGCCCGGGCA
GCGTGGATAACCTGCAGTATAATGTATTTAACTCCTTTAAGCATTGTATTAACAAAACAA
GTTGTATGCTAAGTATCATTAGATGAGGAAAGTTATCAAATGCCTGGAGAGGAGATTAGA
CTAGACACTCGAAATCCAACAGTGATTTGTTTGTAAACTCGGATCTCAGAGATTACCCGG
TACACAAATTGACGCTTAGACAACTTAATAACACATTGCGGACGTTTTTAATGTACTGGG
GTGGTTTTTCTTTTCACCAGTGAGACGGGCAACAGCTGATTGCCCTTCACCGCCTGGCCC
TGAGAGAGTTGCAGCAAGCGGTCCACGCTGGTTTGCCCCAGCAGGCGAAAATCCTGTTTG
ATGGTGGTTCCAAATCGGGCAAAAT
   
Sequence Name 38-K116054-SALK_012199C-R210
GenBank Accession LN485267 [GenBank]
Sequencing primer T-DNA primer, R210: 5'-CCCAGTACATTAAAAACGTCCGC-3'
Sequence (A. th genome BLAST matches underlined) 
>38-K116054-SALK_012199C-R210
CTGAAATCCGAGTTTACAAACAACTCACTGTGGTATTTCAAGTGTCTAGTCTAATCTCCT
CTCCCGGTATTTGATAACTTTCCTCATCTAATGATACTTAGCATACAACTTGTTTTGTTA
ATACAATGCTTAAAGGAGTTAAATACATTATACTGCAGGTTATCCACGCTGCCCGGGCAA
ATGCTACTCATAACCATGGACTAGATCCTGACCGTCTCCTTGTTGGTATGTAAAACTGAT
TCTGGATCCCTGATTTCCTTGTTTTACATTTAAAAAGAGAACGTGATATTTTAGAGAGTT
CGCCGATTGGTACTTTAAGGAAGCAAACATGATATGCCAGAACGATGTATTTCATCTAAG
CTTGTGATATGTGATTGCAGCGGAAGCATTTGTTGGGAAGGGACTGTTTGGGAAGAAGGT
ATCTTACCCTGCAAAAGGAAGAAGCGGGATTATATCAATAACTTTTACTTCCCC

Last Updated on 06.12.2018 16:11