Sequences from confirmation amplicon


Confirmation sequences for line SALK_012424c and hit At2g20430 are shown.
Line ID SALK_012424c
Locus At2g20430 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr2:8809078
Primers used for PCR 29vi - LBb3, show primer details
   
Sequence Name 39-K117945-SALK_012424C-29vi
GenBank Accession LN485286 [GenBank]
Sequencing primer gene specific primer, 29vi: 5'-GAAGAAATACAAATCGGAAATCCA-3'
Sequence (A. th genome BLAST matches underlined) 
>39-K117945-SALK_012424C-29vi
ATATCGGTTGGGATGGACCATCCGCTAATGCCACCGCACCAAGCTGGGTATGTTTTTACT
CTTTTCTACTTCTTTGGTAATTCTTAAAAGGCGTAAACAATAATCGTGATAAAATAGGCA
AATGAAAGATTGTTTTATTATCCTACGCATATTTGGATGGATGTGAGCTCTAGATTAATA
CTTGACTCAAATCTACGAGGTCATATATATTAAAAATTTGACCTAAAACGTTTTCAGAAA
ATGTCGAATTGATTTGGTTAATGTTATGTGATTTGAAAGATGACTGAGTTCAACTCCGGC
GGGGGATTCGAATCTGCTGAAGGAGTCGGAGAAACAAATTGACGCTTAGACAACTTAATA
ACACATTGCGGACGTTTTTAATGTACTGGGGTGGTTTTTCTTTTCACCAGTGAGACGGGC
AACAGCTGATTGCCCTTCACCGCCTGGCCCTGAGAGAGTTGCAGCAAGCGGTCCACGCTG
GTTTGCCCCAGCAGGCGAAAATCCTGTTTGATGGTGGTTCCGAATCGAGCAAAAT
   
Sequence Name 04-K117944-SALK_012424C-R210
GenBank Accession LN484384 [GenBank]
Sequencing primer T-DNA primer, R210: 5'-CCCAGTACATTAAAAACGTCCGC-3'
Sequence (A. th genome BLAST matches underlined) 
>04-K117944-SALK_012424C-R210
GACTCCTTCGCAGATTCGAATCCCCCGCCGGAGTTGAACTCAGTCATCTTTCAAATCACA
TAACATTAACCAAATCAATTCGACATTTTCTGAAAACGTTTTAGGTCAAATTTTTAATAT
ATATGACCTCGTAGATTTGAGTCAAGTATTAATCTAGAGCTCACATCCATCCAAATATGC
GTAGGATAATAAAACAATCTTTCATTTGCCTATTTTATCACGATTATTGTTTACGCCTTT
TAAGAATTACCAAAGAAGTAGAAAAGAGTAAAAACATACCCAGCTTGGTGCGGTGGCATT
AGCGGATGGTCCATCCCAACCGATATGGGCAACATGTTTTACGTCCGTTGGATTTCCGAT
TTGTATTTCTTC

Last Updated on 06.12.2018 16:11