Sequences from confirmation amplicon


Confirmation sequences for line SALK_012841c and hit At4g17980 are shown.
Line ID SALK_012841c
Locus At4g17980 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr4:9979080
Primers used for PCR 30dh - LBb3, show primer details
   
Sequence Name 24-K109819-SALK_012841C-30dh
GenBank Accession LN484972 [GenBank]
Sequencing primer gene specific primer, 30dh: 5'-GCAGTCAATTGCTCTATCACTGAG-3'
Sequence (A. th genome BLAST matches underlined) 
>24-K109819-SALK_012841C-30dh
TAGACTATATATCAAAAGTATTTCGTAGATCATTTTCAAGAGGAAGAAATATTCTTATAA
ATTTTAAAAGAATGAAATGTAGAAAGCTCATGGAAAAAAGAATTTTGTTTGAAAGGTCTC
TAACAATTGAGAAATGCAAGCTTTTGGGATTTTCATACATCTGTAAACAAATTGCTGCGT
CACATAGTTTTTGATTTTAATCTCTGCATATTTATGGAATTTTGTTTACATAAACTTTTG
AAATGTCAAAATGGTCCCGAAACTATTGCTTTTATAGACAAAATATTGGATTTTTAATGC
AAATGATTGTTATGACTTCTACATAAGTTGCTTTATATAATATAAGTTTGGTCCAATTAA
TGATTGAGAAGAACATGATTGATTCTCTTTGCATATATCATATCAAAGGTATCGGAACAT
TAAAATAAGACTCTATAATAACGTAAATCTTCATACGAAAAAACTTTAAATAAGGAATTT
ATTATACTCATTCCTTATATTAGCAAACAATATATATTAAACTAAAATAAAAACCAAGAG
TAAGATAATTAAATTCTCAAAAGAAAATGATAGACGTTCACAACTAGTTCTTCTTCTTCT
TCCAAGTTCCAAGACACCAACGTTAAAAGGCGTTTTTGTCCTAAGAACGAACCAACATTT
CTTGTAGCTTAAAAAGGTTTGAGTAGTCAAATGAACCGGGTTTGGTCATTT
   
Sequence Name 08-K109818-SALK_012841C-R210
GenBank Accession LN484487 [GenBank]
Sequencing primer T-DNA primer, R210: 5'-CCCAGTACATTAAAAACGTCCGC-3'
Sequence (A. th genome BLAST matches underlined) 
>08-K109818-SALK_012841C-R210
GTCATTTGTTACACCACAATATATCCTCAGATTGAGGAGGTTGCTTCTGAGTGCTTTCCC
AACTACCATTTTCCGGTCACCACGGCAAACCACCACGTGGAATTTCCGGTGAGCTCTTGT
TATTTCAATGTGGACCAGGACATTGATCAGTCGATGCAAACCGGTTATTGGACAAATTAT
GAAAATGACCAAACCGGTTCATTTGACTACTCAAACCTTTTTTAGCTACAAGAAATGTTG
GTTCGTTCTTAGGACAAAAACGCCTTTTAACGTTGGTGTCTTGGAACTTGGAAGAAGAAG
AAGAACTAGTTGTGAACGTCTATCATTTTCTTTTGAGAATTTAATTATCTTACTCTTGGT
TTTTATTTTAGTTTTATATATATTGTTTGCTAATATAAGGAATGAGTATAATAAATTCCT
TATTTAAAGTTTTTTCGTATGAAGATTTACGTTATTATAGAGTCTTATTTTAATGTTCCG
ATACCTTTGATATGATATATGCAAAGAGAATCAATCATGTTCTTCTCAATCATTAATTGG
ACCAAACTTATATTATATAAAGCAACTTATGTAGAAGTCATAACAATCATTTGCATTAAA
AATCCAATATTTTGTCTATNAAAGCAATAGTTTCGGGACCATTTTGACATTTCAAAAGTT
TATGTAAACAAAATTCCATAAATATGCAGAGATTAAAATCAAAAACTATGTGACGCAGCA
ATTTGTTTACAGATGTATGAAAATCCCAAAAGCTTGCATTTCTCAATTTGTAGAGACCTT
TCAAACAAAATTCTTTTTTCCATGAGCTTTCTACATTTC

Last Updated on 06.12.2018 16:11