Sequences from confirmation amplicon


Confirmation sequences for line SALK_012997c and hit At1g62300 are shown.
Line ID SALK_012997c
Locus At1g62300 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:23018227
Primers used for PCR AW93 - LBb3, show primer details
   
Sequence Name 25-K109819-SALK_012997C-AW93
GenBank Accession LN484999 [GenBank]
Sequencing primer gene specific primer, AW93: 5'-GATTTCGTGTGAAGAAGGAAGAAC-3'
Sequence (A. th genome BLAST matches underlined)
>25-K109819-SALK_012997C-AW93
TAGTCGACTTTCGATAATCATATGAAATACAATTTTCTTACTAATGGTTTGTATTAGTGT
GGACGTGTCATAATTTGGTTTACATTTGAAATATCAAATAAAAATACCAAATTTTGTTTC
ATTTTTTTTTTTTGAAAAAGGGAACCAAATTTGGTTTCCTCCCAAAAGGATTCCAAATTT
GAAATTTAATTTGGTAAAAACCGGGTTGAAACCCCCAACCACCGGGAAAACCAAAAGGGG
AGAACCCAAGGACCAAGAAGGAAAAACCCCCCAAAAGGAAAAAAAACCGGCCAAAAAAGG
GGGAAAGTTAATTTGGTTTTCCCTTAGGAAATTTTAAAAAAAGAAAAATTTGAACCCGGT
TGAATAAAAATTCCAATTTTTTTTGGTTAACCCCGGTGGGGAAATTCCCAAAAGGGTTGA
AAAAAAAGGCCAGGGGAAACCCAAACCCTAAAAAATTGGCTCCCCCAGTTTGCCACCGGT
TCCACCTAAAAACCCCTTGGCCCCTAAACAACCTAAAAACCCCTTGGGGAAGTTTTTAAA
GGACCGGGGGGGGTTTTCCTTTCCCCCGGGGAAACGGGAAACGCTGAATGGCCCTCCCCC
CCCGGCCCCAAAAAAATTTCCACCAAGCGCCCCCCCCGGTTTGCCCCAACCGGGCAAAAA
CCCGGTTGGAGGGGGGTCCCAAAACCCGAAAAAA
   
Sequence Name 09-K109818-SALK_012997C-R210
GenBank Accession LN484517 [GenBank]
Sequencing primer T-DNA primer, R210: 5'-CCCAGTACATTAAAAACGTCCGC-3'
Sequence (A. th genome BLAST matches underlined)
>09-K109818-SALK_012997C-R210
GTTATTAGTTGTACTGTTGCTAACTTGTGTAAGCAATTCTCTAAGCTTTTGATTATCCAT
TGTCATTTTCTTCAACTCATCTTGTAATTTCACCAACTGTGATAAACAAAAAAAAATCGA
AATATTAATCAAACCGGTTCAAATTATTCATCTATTAAATATTACTAATGTAAAATCAAA
CTAAACTTACCTCATTTTTCGCACGCTTATCTTCCATTTCGGAAGATTCTCCATCATCGA
TCATTGACTCATCACTCTTTGTATTACCAGTTGTTCGAAGATTCAAACCGGTCTATAACA
AATTAAAATTCAAATTTAGAAATCATTTTGTAAGAAAACAAAATATGATACCTTTATCAA
AAAAAAAAAATTAAACCAAATTTGGGTTTTTTTTTTGAATTTTCCAATGGAAACCCAATT
TTTAACCCTCCCCCCTTAATACAACCCTTTATAAAAAAATTGGTTTTCCAAATAATTTAC
CAAAAAGCCGAATTTACTTTACCTCCCGTCCAACAATTTGGTTCTCCCTT
Primers used for PCR 68wt - R212, show primer details
   
Sequence Name 26-K118859-SALK_012997C-68wt
GenBank Accession LN485049 [GenBank]
Sequencing primer gene specific primer, 68wt: 5'-GTTTCCCTCGTAGGTTGTAATCAG-3'
Sequence (A. th genome BLAST matches underlined)
>26-K118859-SALK_012997C-68wt
AACCTATTACCCAATCACGCAAACATATGTACGTTGAAATCTCTAATGTTTTTACCACAA
GAGATCTTTTAGTCCAATGGTTAAATCTTTTAAATCTTACTTGTTTGCGAACGGGACAGC
CCGTGGCCATCGTGCAGCGGTAATATGCCCGCGGACAAGGATTCCCTTTGGCCATCTTCT
GGCCATATTTTCTCCATTGACATCCATCGCTTATCTATAGAAACATAAAGTACAAAGATA
ATTAATCTCTAAAGCACATAGTTTATTTTTTATATAGTCTTATAAGTATTAGTATCTAAT
AAATCAACTTACCATCGGAGCTTCCGATCGGGCACGAACGGAGACACGGGCTTTCCTCAT
CGTAGCTTCAGCGGTTTGATCAAATGTCGTCGGGGTAGTAGAATTCACCTTCTGAATTTT
GTTGGACTCAGTTTCGGGGCTTTCTTCACGCCCAAGTCTCTTCCCGTTACTACGCCTCTC
GGCTGCAGAAGAACCCCCCGAACGAGTTCTATCTTCGGATGAAGAATTTGACACATCCTC
GGCCTCACCTACTGCTCTCGTAGGGCCTAAATCAATAAATTGCCTTGGTACTATCGTCTC
CTCAGGCTTCTCAGCAGCTTCTATTACCTAATCATATAGGTTCACCAAAATTAATTGCCT
ATTCATGTAGGGTAAATTCCATTTACTTGTGGTTGATCAATCAAACACTGATAGTTTAAA
CTGAAGGCGGGAAACGACAATCTGATCATGAGCGGAGAATTAAGGGAGTCACGTTATGAC
CCCCGCCGATGACGCGGGACAAGCCGTTTTACGTTTGGAACTGACAGAACCGCAACGTTT
AAGGAGCCACTCAGCCGCGGGTTTCTGGAGTTTATG
   
Sequence Name 02-K118858-SALK_012997C-R214
GenBank Accession LN484323 [GenBank]
Sequencing primer gene specific primer, R214: 5'-AACGTTGCGGTTCTGTCAGTTCC-3'
Sequence (A. th genome BLAST matches underlined)
>02-K118858-SALK_012997C-R214
GGGGACATAACGTGACTCCCTTAATTCTCCGCTCATGATCAGATTGCTTTTTCCCGCCTT
CAGTTTAAACTATCAGTGTTTGATTGATCAACCACAAGTAAATGGAATTTACCCTACATG
AATAGGCAATTAATTTTGGTGAACCTATATGATTAGGTAATAGAAGCTGCTGAGAAGCCT
GAGGAGACGATAGTACCAAGGCAATTTATTGATTTAGGCCCTACGAGAGCAGTAGGTGAG
GCCGAGGATGTGTCAAATTCTTCATCCGAAGATAGAACTCGTTCGGGGGGTTCTTCTGCA
GCCGAGAGGCGTAGTAACGGGAAGAGACTTGGGCGTGAAGAAAGCCCCGAAACTGAGTCC
AACAAAATTCAGAAGGTGAATTCTACTACCCCGACGACATTTGATCAAACCGCTGAAGCT
ACGATGAGGAAAGCCCGTGTCTCCGTTCGTGCCCGATCGGAAGCTCCGATGGTAAGTTGA
TTTATTAGATACTAATACTTATAAGACTATATAAAAAATAAACTATGTGCTTTAGAGATT
AATTATCTTTGTACTTTATGTTTCTATAGATAAGCGATGGATGTCAATGGAGAAAATATG
GCCAGAAGATGGCCAAAGGGAATCCTTGTCCGCGGGCATATTACCGCTGCACGATGGCCA
CGGGCTGTCCCGTTCGCAAACAAGTAAGATTTAAAAGATTTAACCATTGGACTAAAAGAT
TCTTGTTGTAAAAACATTAGAGATTTCAATGTATATATGTTTGCGTGATTGGCTAATACG
TTCGGTCCGAAATGATATTTATAGGTTCAACGTTGCGCGGAAGACAGATCAATTCTGATT
ACACCT

Last Updated on 06.12.2018 16:11