Sequences from confirmation amplicon


Confirmation sequences for line SALK_013217c and hit At3g25750 are shown.
Line ID SALK_013217c
Locus At3g25750 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:9401233
Primers used for PCR 49jd - LBb3, show primer details
   
Sequence Name 19-K113259-SALK_013217C-49jd
GenBank Accession LN484819 [GenBank]
Sequencing primer gene specific primer, 49jd: 5'-TCTTCAAACATCCATGGTACTCAG-3'
Sequence (A. th genome BLAST matches underlined) 
>19-K113259-SALK_013217C-49jd
CAGCACAATGAGTGTGTTGTCTCCCAAGCAACTAACCTCAACCCATTTTGCCAAATCCTC
GTCCATCTTATAAACCTTAAAACCAACGGTCCTCTGGATATGATTTCGAGTTATGCTAAG
TTCATGAACAATGCATAGATCTCCACAATATTCCACGAGCCTCGTGTCTTGGCAACTATC
ATATTTATAGTAGTCCAACGGAGTTGAAGATGTCTGTTGAACAATGGTAAGCTGTGATAA
ACTAATCCACCAAATTGCTCCTTTTAAGTCCACGGCATAGATACGTCCCATGTGAAGTAT
AATGTCCGAGAAATCTTCGACTTGGTTTTTAATTTTTGTCCAACTTCTACTTCCTTCTCT
AGTCTTACAACACCATATTTTATTATCGTGGTAATTGACTGCAAAAAAAATATTGTCTAG
GAAAGCAACTCTAAAGAATACACCTGATGGTATTTTGTCACTATTCTTGATATAATTAGA
GAATATCTCTGAATCTAATGGTATCTTTTCGTCCTTCAATCCATCGAATATTTGAATCTC
GTAAGATTGGCGAATCTCTGAAACGCCAACCTTCAAAAGGTCTAGGGTTTGGTGGGAACG
GGTGATGCGCTCGCCAGAGAGAGGACACAAGAGAGTAATCTTACGATACATTTTGGAGTA
AACAAATTGACGCTTAGACAACTTAATAACACATTGCGGACGTTTTTAATGTACTGGGGT
GGTTTTTCTTTTCACCAGTGAGACGGGCAACAGCTGATTGCCCTTCACCGCCTGGCCCTG
AGAGAGTTGCAGCAAGCGGTCCACGCTGGTTTGCCCCAGCAGGCGAAAATCCTGTTTGAT
GGTGGTTCC
   
Sequence Name 02-K113258w-SALK_013217C-R210
GenBank Accession queueing for submission to ENA/GenBank
Sequencing primer T-DNA primer, R210: 5'-CCCAGTACATTAAAAACGTCCGC-3'
Sequence (A. th genome BLAST matches underlined) 
>02-K113258w-SALK_013217C-R210
TTTGTTACTCCAAATGTATCGTAAGATTACTCTCTTGTGTCCTCTCTCTGGCGAGCGCAT
CACCCGTTCCCACCAAACCCTAGACCTTTTGAAGGTTGGCGTTTCAGAGATTCGCCAATC
TTACGAGATTCAAATATTCGATGGATTGAAGGACGAAAAGATACCATTAGATTCAGAGAT
ATTCTCTAATTATATCAAGAATAGTGACAAAATACCATCAGGTGTATTCTTTAGAGTTGC
TTTCCTAGACAATATTTTTTTTGCAGTCAATTACCACGATAATAAAATATGGTGTTGTAA
GACTAGAGAAGGAAGTAGAAGTTGGACAAAAATTAAAAACCAAGTCGAAGATTTCTCGGA
CATTATACTTCACATGGGACGTATCTATGCCGTGGACTTAAAAGGAGCAATTTGGTGGAT
TAGTTTATCACAGCTTACCATTGTTCAACAGACATCTTCAACTCCGTTGGACTACTATAA
ATATGATAGTTGCCAAGACACGAGGCTCGTGGAATATTGTGGAGATCTATGCATTGTTCA
TGAACTTAGCATAACTCGAAATCATATCCAGAGGACCGTTGGTTTTAAGGTTTATAAGAT
GGACGAGGATTTGGCAAAATGGGTTGAGGTTAGTTGCTTGGGAGACAACACACTCATTGT
TGCTTGCAACAGTTGTTTTACTGTCGTAGCCTCTGAGTACCATG

Last Updated on 06.12.2018 16:11