Sequences from confirmation amplicon


Confirmation sequences for line SALK_013618c and hit At5g15790 are shown.
Line ID SALK_013618c
Locus At5g15790 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:5150789
Primers used for PCR 34wm - LBb3, show primer details
   
Sequence Name 32-K118709-SALK_013618C-34wm
GenBank Accession LN485177 [GenBank]
Sequencing primer gene specific primer, 34wm: 5'-TGTTGTTTTCTCTCAAGAGGGTTT-3'
Sequence (A. th genome BLAST matches underlined) 
>32-K118709-SALK_013618C-34wm
TTTCTCATTTCCCCATCATATACAATATCATATATTGTTAAAAAATATATTCACATCTCT
CTGCTGCCTTGTCAATCTCCCCAGGTCCGGTCTAGGCAGTACAAGACAGGATCAATGGCA
ACAAGTTACGGTGTTTCGTGGAACTCCATCACCTGCAAATGAGACGAATTCACAGCAGAG
AAAAAAATATTCTTTAGTCAACATGAATGAGAAATAATTCAAATGTTCTGAGTTTCAGGA
AGAATGATTAGCCATATTTGTCATAGACAAGACAAGTAAAGATTTTACGCATGTGCTTCT
ACAAATTGACAAATTGACGCTTAGACAACTTAATAACACATTGCGGACGTTTTTAATGTA
CTGGGGTGGTTTTTCTTTTCACCAGTGAGACGGGCAACAGCTGATTGCCCTTCACCGCCT
GGCCCTGAGAGAGTTGCAGCAAGCGGTCCACGCTGGTTTGCCCCAGCAGGCGAAAATCCT
GTTTGATGGTGGTTCCGAATCGGGCAAAA
   
Sequence Name 07-K118708-SALK_013618C-R210
GenBank Accession LN484476 [GenBank]
Sequencing primer T-DNA primer, R210: 5'-CCCAGTACATTAAAAACGTCCGC-3'
Sequence (A. th genome BLAST matches underlined) 
>07-K118708-SALK_013618C-R210
TTTGTCATTTGTAGAAGCCATGCGTAAAATCTTTACTTGTCTTGTCTATGACAAATATGG
CTAATCATTCTTCCTGAAACTCAGAACATTTGAATTATTTCTCATTCATGTTGACTAAAG
AATATTTTTTTCTCTGCTGTGAATTCGTCTCATTTGCAGGTGATGGAGTTCCACGAAACA
CCGTAACTTGTTGCCATTGATCCTGTCTTGTACTGCCTAGACCGGACCTGGGGAGATTGA
CAAGGCAGCAGAGAGATGTGAATATATTTTTTAACAATATATGATATTGTATATGATGGG
GAAATGAGGAAACGTCTCTCTGCACGTTTGGCTTATGGAGTTCTGAGGAAACCCTCTTGA
GAGAAAACAACAATTNNNTNTTTATAAAAAGCAGGATTC

Last Updated on 06.12.2018 16:11