Sequences from confirmation amplicon


Confirmation sequences for line SALK_014538 and hit At1g73080 are shown.
Line ID SALK_014538
Locus At1g73080 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:27484853
Primers used for PCR 17ce - LBb3, show primer details
   
Sequence Name 17-K108893-SALK_014538C-17ce
GenBank Accession LN484740 [GenBank]
Sequencing primer gene specific primer, 17ce: 5'-CAGAGTAGTGAGATTCCCCAAAAG-3'
Sequence (A. th genome BLAST matches underlined) 
>17-K108893-SALK_014538C-17ce
ACACTACTTGTTCCTGTGCAAATAAAGAATCTGCAGACTACTGCTATTCCCAATCGACTC
AGGGATGTTACCAGAGAACTGATTCGCATACATACTCAGCTCCACAAGCTCCTTAGCATC
ACCAATACTTTGAGGAATCGGACCGGTGAGATTGTTATAGTCAAGATATAAAACCTGCAG
CTTCGGAATTCGAAACAAGGATTCAGGTAACTCACCAGTGAGGAAGTTTATGTAAAGATA
AAGCACCTCCAACCTCTTCAAGCTATCGAGAGTATCTGGGATCTTATCAGAGAATCCATT
TTCAGACAAATCTAGAGTAGCGAGTTTGGTACAGTTTCCTAAAGTGGAAGGTATAGACAA
ATTGACGCTTAGACAACTTAATAACACATTGCGGACGTTTTTAATGTACTGGGGTGGTTT
TTCTTTTCACCAGTGAGACGGGCAACAGCTGATTGCCCTTCACCGCCTGGCCCTGAGAGA
GTTGCAGCAAGCGGTCCACGCTGGTTTGCCCCAGCAGGCGAAAATCCTGTTTGATGGTGG
TTCCGA
   
Sequence Name 01-K108892-SALK_014538C-R210
GenBank Accession LN484271 [GenBank]
Sequencing primer T-DNA primer, R210: 5'-CCCAGTACATTAAAAACGTCCGC-3'
Sequence (A. th genome BLAST matches underlined) 
>01-K108892-SALK_014538C-R210
GTCATTTGTCTATCCTTCACTTTAGGAAACTGTACCAAACTCGCTACTCTAGATTTGTCT
GAAAATGGATTCTCTGATAAGATCCCAGATACTCTCGATAGCTTGAAGAGGTTGGAGGTG
CTTTATCTTTACATAAACTTCCTCACTGGTGAGTTACCTGAATCCTTGTTTCGAATTCCG
AAGCTGCAGGTTTTATATCTTGACTATAACAATCTCACCGGTCCGATTCCTCAAAGTATT
GGTGATGCTAAGGAGCTTGTGGAGCTGAGTATGTATGCGAATCAGTTCTCTGGTAACATC
CCTGAGTCGATTGGGAATAGCAGTAGTCTGCAGATTCTTTATTTGCACAGGAACAAGTTA
GTTGGTTCATTACCTGAAAGTCTCAATCTTTTGGGGAATCTCACTACTCTG

Last Updated on 06.12.2018 16:11