Sequences from confirmation amplicon


Confirmation sequences for line SALK_014625 and hit At3g02780 are shown.
Line ID SALK_014625
Locus At3g02780 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:603024
Primers used for PCR 07hi - LBb3, show primer details
   
Sequence Name 75-K114279-SALK_014625C-07hi
GenBank Accession LN486134 [GenBank]
Sequencing primer gene specific primer, 07hi: 5'-TCGACTCTGTTTCCTTGTTATCCT-3'
Sequence (A. th genome BLAST matches underlined) 
>75-K114279-SALK_014625C-07hi
CTCACAACTTTGTGACATTCATTCATNAATTAAGATAAAAACGAAAACTTTATTATTGCC
TGAGGTGCTTGGAAGTTTTGTAAGTGTCTCACATATCCCAAATTATTATCATCGAAAAGA
TAAAAATGATGAAGAGAAAGTTGATTGATTTAAAAACTTTAAAAAAAGATGTTCAGAGTT
TGTGGATGGTTTTCATGTCTATAGCTTCAACCAAAGTTCCTTTCTCAACATGATCCCACC
ACTTCATCAAGAAATTGTCCACCACCAATCTGAACCATGGTGACAGTTTCAAACCTTCCT
CACCTGCATCTGCTTTCTTCACCAGCTCCTTCAGCTCTTCCCGGCTCACATACTTGATCT
CAGCTACTTCATCTGGGTTTGGTTGAACCTTCACGTCTCGCACGATGAAGAGCAAGTAAT
CAACTGCAAAAGGAAGGCCAACACAGAAGAAGCTTAAGGTTTTATTATTCTGTTCAAGAA
AATGGGAACCAAGAGTCTTGAGAAATGGTATTTACGTTCATGCTCTCCCCATTTGCCATC
AGAAGGAGCCTTGTACAGCATACGTCCCAAGGGAGTGAACTCATCGACTGGTACATCTTC
AGCTACAATACCAAGCTCACAAATATACAAATTGACGCTTAGACAACTTAATAACACATT
GCGGACGTTTTTAATGTACTGGGGTGGTTTTTCTTTTCACCAGTGAGACGGGCAACAGCT
GATTGCCCTTCACCGCCTGGCCCTGAGAGAGTTGCAGCAAGCGGTCCACGCTGGTTTGCC
CCAGCAGGCGAAAATCCTGTTTGATGGTGGTTCCG
   
Sequence Name 61-K114278-SALK_014625C-R210
GenBank Accession LN485771 [GenBank]
Sequencing primer T-DNA primer, R210: 5'-CCCAGTACATTAAAAACGTCCGC-3'
Sequence (A. th genome BLAST matches underlined) 
>61-K114278-SALK_014625C-R210
CGTCATTTGTAATTTGTGAGCTTGGTATTGTAGCTGAAGATGTACCAGTCGATGAGTTCA
CTCCCTTGGGACGTATGCTGTACAAGGCTCCTTCTGATGGCAAATGGGGAGAGCATGAAC
GTAAATACCATTTCTCAAGACTCTTGGTTCCCATTTTCTTGAACAGAATAATAAAACCTT
AAGCTTCTTCTGTGTTGGCCTTCCTTTTGCAGTTGATTACTTGCTCTTCATCGTGCGAGA
CGTGAAGGTTCAACCAAACCCAGATGAAGTAGCTGAGATCAAGTATGTGAGCCGGGAAGA
GCTGAAGGAGCTGGTGAAGAAAGCAGATGCAGGTGAGGAAGGTTTGAAACTGTCACCATG
GTTCAGATTGGTGGTGGACAATTTCTTGATGAAGTGGTGGGATCATGTTGAGAAAGGAAC
TTTGGTTGAAGCTATAGACATGAAAACCATCCACAAACTCTGAACATCTTTTTTTAAAGT
TTTTAAATCAATCAACTTTCTCTTCATCATTTTTATCTTTTCGATGATAATAATTTGGGA
TATGTGAGACACTTACAAAACTTCCAAGCACCTCAGGCAATAATAAAGTTTTCGTTTTTA
TCTTAATTAATGAATGAATGTCACAAAGTTTGTGAGATGTACAAAAAGCGTCGAGGAAAA
GGATAACAAGGA

Last Updated on 06.12.2018 16:11