Sequences from confirmation amplicon


Confirmation sequences for line SALK_014639c and hit At2g43240 are shown.
Line ID SALK_014639c
Locus At2g43240 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr2:17971839
Primers used for PCR 13ty - LBb3, show primer details
   
Sequence Name 23-K117180-SALK_014639C-13ty
GenBank Accession LN484957 [GenBank]
Sequencing primer gene specific primer, 13ty: 5'-CTCTCCTGGGTTCTGAATGTAAAG-3'
Sequence (A. th genome BLAST matches underlined) 
>23-K117180-SALK_014639C-13ty
GTGCGATANCAACTTCCTCGGAAACTAGGAACTGTTATATATAAAGGTATGGTTATATTA
ACATGGCAGAGAAGAATACGATAAATATTTAAGTAGAATGCAAAAAAAAGCTTCTTAAAT
TAAATCCTGTTAAACGCAGTACTCACAATCTTGCCACTGTGTCGACAGGTCCTGGCAGTT
TTGACATCCTACAAGGACATTCTAGGGCTACTATGTTTCTGATACTGAACAACGCAGCAC
AAGGGATTCTATCCTCCTTTTTCTTCAAATATGCAGGTAAAAATAACACTCAACTTTGTT
ACCTCAGTTAATTTATGGATTAGTTCTGGATATGGTGGTTCAATAAATTAAATTTGAATT
TTGCTATACTTGGAAAATATAAACCGTTCAGTAAGGTTGTATTCTAAGATATTTAGTTGC
CTTTGTGTCCATATGTTCTCAGATACAATCTTGAAGAAATATTCGTCCACAGTAGCCACA
ATATTTACTGGCATAGCATCTGCAGCACTCTTAGACAACTTAATAACACATTGCGGACGT
TTTTAATGTACTGGGGTGGTTTTTCTTTTCACCAGTGAGACGGGCAACAGCTGATTGCCC
TTCACCGCCTGGCCCTGAGAGAGTTGCAGCAAGCGGTCCACGCTGGTTTGCCCCAGCAGG
CGAAAATCCTGTTTGATGGTGGTTC
   
Sequence Name 02-K117179-SALK_014639C-R210
GenBank Accession LN484317 [GenBank]
Sequencing primer T-DNA primer, R210: 5'-CCCAGTACATTAAAAACGTCCGC-3'
Sequence (A. th genome BLAST matches underlined) 
>02-K117179-SALK_014639C-R210
TATTGTGGCTACTGTGGACGAATATTTCTTCAAGATTGTATCTGAGAACATATGGACACA
AAGGCAACTAAATATCTTAGAATACAACCTTACTGAACGGTTTATATTTTCCAAGTATAG
CAAAATTCAAATTTAATTTATTGAACCACCATATCCAGAACTAATCCATAAATTAACTGA
GGTAACAAAGTTGAGTGTTATTTTTACCTGCATATTTGAAGAAAAAGGAGGATAGAATCC
CTTGTGCTGCGTTGTTCAGTATCAGAAACATAGTAGCCCTAGAATGTCCTTGTAGGATGT
CAAAACTGCCAGGACCTGTCGACACAGTGGCAAGATTGTGAGTACTGCGTTTAACAGGAT
TTAATTTAAGAAGCTTTTTTTTGCATTCTACTTAAATATTTATCGTATTCTTCTCTGCCA
TGTTAATATAACCATACCTTTATATATAACAGTTCCTAGTATTCCGAGGAAGTTGAATAT
CGCACCATAACCATAAAGAAATAAGTTCTGCACAAGGGAAATTAAAGCTTTACATTCAGA
ACCCAAGGAGAGAANNTTTTTTT

Last Updated on 06.12.2018 16:11