Sequences from confirmation amplicon


Confirmation sequences for line SALK_015052 and hit At5g52860 are shown.
Line ID SALK_015052
Locus At5g52860 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:21420893
Primers used for PCR SC12 - R204, show primer details
   
Sequence Name 50-K106282-SALK_015052C-R210
GenBank Accession LN485493 [GenBank]
Sequencing primer T-DNA primer, R210: 5'-CCCAGTACATTAAAAACGTCCGC-3'
Sequence (A. th genome BLAST matches underlined) 
>50-K106282-SALK_015052C-R210
GACTGTTGTGTACCCGGAAGACTTGACTCCCTCGAAGGTTTCTTGCTCTTCAAGGGATTT
ACCGTCCCTCCGCAGCTCAATTCCCTTGAATATGCAATGGAAATACTTCAAGAACTACGA
GAATCCGACGGAAACACCGATGCTACTGCTCTCCCTTCAATCGAAAACCGGAAACAGAGA
GAAAAACAGAGTATTGTTCGATATAGAAAATCAAGAATCACCGAGATAAGCCTTCTCGCG
AGAAGATTCTGGAAGATCATTTACCGGACAAGGCAACTACTTCTAACCAATGCATTAGAA
GCCCTTGTAGTTGGTCTTGTCCTAGGAACTATCTACATCAACATCGGAATCGGCAAAGCA
GGAATTGAGAAAAGATTCGGTATGTTCGCCTTCACGCTCACTTTTCTCCTCTCTTCAACA
ACCGAAACCCTCCCGATCTTCATCAACGAGCGTCCAATTCTTCTCAGGGAGACTTCGAGT
GGTATTTACAGACTCTCCTCTCACATTCTCGCAAATACACTCGTATTCTTGCCGTACTTG
TTCGTTATCTCAATCATTTACTCTGTTTCGGTCTATTTCCTCATGGNTCTCTG
   
Sequence Name 66-K106283-SALK_015052C-SC12
GenBank Accession LN485888 [GenBank]
Sequencing primer gene specific primer, SC12: 5'-GCAGAGACCAATGAGGAAATAGAC-3'
Sequence (A. th genome BLAST matches underlined) 
>66-K106283-SALK_015052C-SC12
TACGAACAAGTACGGCAAGAATACGAGTGTATTTGCGAGAATGTGAGAGGAGAGTCTGTA
AATACCACTCGAAGTCTCCCTGAGAAGAATTGGACGCTCGTTGATGAAGATCGGGAGGGT
TTCGGTTGTTGAAGAGAGGAGAAAAGTGAGCGTGAAGGCGAACATACCGAATCTTTTCTC
AATTCCTGCTTTGCCGATTCCGATGTTGATGTAGATAGTTCCTAGGACAAGACCAACTAC
AAGGGCTTCTAATGCATTGGTTAGAAGTAGTTGCCTTGTCCGGTAAATGATCTTCCAGAA
TCTTCTCGCGAGAAGGCTTATCTCGGTGATTCTTGATTTTCTATATCGAACAATACTCTG
TTTTTCTCTCTGTTTCCGGTTTTCGATTGAAGGGAGAGCAGTAGCATCGGTGTTTCCGTC
GGATTCTCGTAGTTCTTGAAGTATTTCCATTGCATATTCAAGGGAATTGAGCTGCGGAGG
GACGGTAAATCCCTTGAAGAGCAAGAAACCTTCGAGGGAGTCAAGTCTTCCGTGGTACAC
AACAGTTCCTTTTGAAAGACAACTTAATAACACATTGCGGACGTTTTTAATGTACTGGGG
TGGTTTTTCTTTTCACCAGTGAGACGGGCAACAGCTGATTGCCCTTCACCGCCNGGCCCT
GAGAGAGTTGCAGC
Primers used for PCR 44xl - R212, show primer details
   
Sequence Name 77-K119133-SALK_015052C-44xl
GenBank Accession LN486182 [GenBank]
Sequencing primer gene specific primer, 44xl: 5'-TGACCCAAAACTCTACCGATATACA-3'
Sequence (A. th genome BLAST matches underlined) 
>77-K119133-SALK_015052C-44xl
CCAAAAGATCTTAGTGAAAGAAAACAACCCAAAAATGTAAAATTAGGGTTTTTGGCTTAC
GACATTTACGTCATCACAAGACCAAATAAAAACTTAATTATATAAATCTCATTCACTACA
CTCTTCAACGTCTTAAACTCTTACAAGTTAGAACTTAGAACCATTCTCTATGGAAATACC
TCCATCACCTCCGCCGGAGACGGCGGCCTACACATTAACCACCTCTTCAATCTCCTACAC
CATACCAAAAACCTCTCTCTCCCTCCTCCGCTTCCCGGCGACTGAGCCACCCTCCTTTAT
CCTCCGCAACATCACTCTCACCGCTCATCCAACCGAGATCCTCGCCGTCGTTGGCCCTTC
CGGCGCCGGAAAATCAACTCTCCTTGACATTCTCGCCTCTAAAACCTCCCCAACTTCCGG
TTCAATCCTCCTTAACTCCATTCCCATTAACCCTTCCTCTTATCGAAAAATCTCCTCTTA
CGTTCCCCAACATGACTCTTTCTTCCCTCTCCTCACCGTCTCCGAGACCTTCTCCTTCGC
TGCTTGTCTCTTGCTCCCAAACCCATCAATAGTTTCTGAAACCGTAACCTCTCTTCTATC
AGAGCTTAACCTAACACATCTTTCCCACACAAGACTCGCTCAAGGCTTATCCGGTGGCGA
AAGAAGAAGAGTCTCGATAGGGTTAAGCCTCCTTCACGACCCTTGCTTTCTTCTCCTCGA
TGAACCCACTTCGGGTTTAGACAGTAAATCCGCTTTTGACGTCATTCACATCCTCAAATC
CATCGCTGTGTCAAGACAACGGAACGTGATATTGTCCATTCAAACACTGATAGTTTAAAC
TGAAGGCGGGGAAACGACAATCTGATCATGAGC
   
Sequence Name 63-K119132-SALK_015052C-R214
GenBank Accession LN485833 [GenBank]
Sequencing primer gene specific primer, R214: 5'-AACGTTGCGGTTCTGTCAGTTCC-3'
Sequence (A. th genome BLAST matches underlined) 
>63-K119132-SALK_015052C-R214
GCGGGGGTCATAACGTGACTCCCTTAATTCTCCGCTCATGATCAGATTGTCGTTTCCCGC
CTTCAGTTTAAACTATCAGTGTTTGAATGGACAATATCACGGTCCGTTGTCTTGACACAG
CGATGGATTTGAGGATGTGAATGACGTCAAAAGCGGATTTACTGTCTAAACCCGAAGTGG
GTTCATCGAGGAGAAGAAAGCAAGGGTCGTGAAGGAGGCTTAACCCTATCGAGACTCTTC
TTCTTTCGCCACCGGATAAGCCTTGAGCGAGTCTTGTGTGGGAAAGATGTGTTAGGTTAA
GCTCTGATAGAAGAGAGGTTACGGTTTCAGAAACTATTGATGGGTTTGGGAGCAAGAGAC
AAGCAGCGAAGGAGAAGGTCTCGGAGACGGTGAGGAGAGGGAAGAAAGAGTCATGTTGGG
GAACGTAAGAGGAGATTTTTCGATAAGAGGAAGGGTTAATGGGAATGGAGTTAAGGAGGA
TTGAACCGGAAGTTGGGGAGGTTTTAGAGGCGAGAATGTCAAGGAGAGTTGATTTTCCGG
CGCCGGAAGGGCCAACGACGGCGAGGATCTCGGTTGGATGAGCGGTGAGAGTGATGTTGC
GGAGGATAAAGGAGGGTGGCTCAGTCGCCGGGAAGCGGAGGAGGGAGAGAGAGGTTTTTG
GTATGGTGTAGGAGATTGAAGAGGTGGTTAATGTGTAG

Last Updated on 06.12.2018 16:11