Sequences from confirmation amplicon


Confirmation sequences for line SALK_015166 and hit At5g57050 are shown.
Line ID SALK_015166
Locus At5g57050 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:23089305
Primers used for PCR 38db - LBb3, show primer details
   
Sequence Name 02-K109680-SALK_015166C-38db
GenBank Accession LN484301 [GenBank]
Sequencing primer gene specific primer, 38db: 5'-ATAAAAGCTGCCTTACACACCACT-3'
Sequence (A. th genome BLAST matches underlined) 
>02-K109680-SALK_015166C-38db
GTATGTATTATTTTGTCTTCATCACCTAAATCTAAACATTCACATGGATTTACAAATTAT
TGTGACGTTTATTCAACCATCCCATATTCTGGTTGGTTTAGTAGATTTAAACAAAATGAA
AAAATTATGGTGATGAAGACTAATGTAATTTTTGGTAATGAAAAAATGATGTCAATACTA
TTGAAACTATATCACTCTAACGAAAACACACACACAAAGAGAAGTTATGAAACATCAAAT
AATAACACTAAATATTTACGTAGACGAATGTAGTGTTATTACAACTAACCCCAAGATAAA
AAAAAATACAAGGGGGCTTTTTGTTTGGAAGTTACACTATTCCCATGTACATTATATATA
TCATGTTAGTAAAATAAATATAAAAAGATTAAATAATGGTGGGAAGACTAAGAAAGTAAA
TAACAAATTTAAGCAGGAAATTAATATTACAATGTAATTATTACCTGTTGTTTTATCTTT
CTTTTTTCACTAAAGAGAATTTTTACCCACCATCAAAACTTTTTTTTTCACTTTTCTTCC
AAACCTTCTTTTTCAATTCAAGGATTTGCTCTTGAATAACACATTGTGGACGTTTTTAAT
GTACTGGGGTGGTTTTTCTTTTCACCAGTGAGACGGGCAACAGCTGATTGCCCTTCACCG
CCTGGCCCTGAGAGAGTTGCAGCAAGCGGTCCACGCTGGTTTGCCCCAGCAGGCGAAAAT
CCTGTTTGATGGTGGTTCCGAAATCCGCAAA
   
Sequence Name 18-K109679-SALK_015166C-R210
GenBank Accession LN484773 [GenBank]
Sequencing primer T-DNA primer, R210: 5'-CCCAGTACATTAAAAACGTCCGC-3'
Sequence (A. th genome BLAST matches underlined) 
>18-K109679-SALK_015166C-R210
GATTGAAAGAAGGTTTGGAAGAAAGTGAAAAAAAAAGTTTTGATGGTGGGTAAAAATTCT
CTTTAGTGAAAAAAGAAAGATAAAACAACAGGTAATAATTACATTGTAATATTAATTTCC
TGCTTAAATTTGTTATTTACTTTCTTAGTCTTCCCACCATTATTTAATCTTTTTATATTT
ATTTTACTAACATGATATATATAATGTACATGGGAATAGTGTAACTTCCAAACAAAAAGC
CCCCTTGTATTTTTTTTTATCTTGGGGTTAGTTGTAATAACACTACATTCGTCTACGTAA
ATATTTAGTGTTATTATTTGATGTTTCATAACTTCTCTTTGTGTGTGTGTTTTCGTTAGA
GTGATATAGTTTCAATAGTATTGACATCATTTTTTCATTACCAAAAATTACATTAGTCTT
CATCACCATAATTTTTTCATTTTGTTTAAATCTACTAAACCAACCAGAATATGGGATGGT
TGAATAAACGTCACAATAATTTGTAAATCCATGTGAATGTTTAGATTGTAGGTGATTGAA
GACAAAATAATACATACTTATATCACAAGTTAATTGTAAAGTGGTGTGTAAGGCAGCTTT
T

Last Updated on 06.12.2018 16:11