Sequences from confirmation amplicon


Confirmation sequences for line SALK_015182c and hit At5g61590 are shown.
Line ID SALK_015182c
Locus At5g61590 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:24765075
Primers used for PCR AD08 - R204, show primer details
   
Sequence Name 55-K106282-SALK_015182C-R210
GenBank Accession LN485613 [GenBank]
Sequencing primer T-DNA primer, R210: 5'-CCCAGTACATTAAAAACGTCCGC-3'
Sequence (A. th genome BLAST matches underlined) 
>55-K106282-SALK_015182C-R210
GCGTCATTTGTTTACCCTATGTCCAGAGATTCTGCAAATGGAAGCAGAATCATCATCATC
ATCATCAACAACAACGTCACCTGAGGTTGAGACTGTCTCAAACCGGAAAAAAACAAAGAG
GTTTGAAGAAACGAGACATTACAGAGGCGTGAGAAGGAGGCCATGGGGGAAATTTGCAGC
AGAGATTCGAGATCCGGCAAAGAAAGGATCCAGGATTTGGTTAGGCACTTTTGAGAGTGA
TATTGATGCTGCAAGGGCTTACGACTATGCAGCTTTTAAGCTCAGGGGAAGAAAAGCTGT
TCTCAACTTTCCTTTGGATGCCGGAAAGTATGATGCTCCGGTCAATTCATGCCGAAAAAG
GAGGAGAACCGATGTACCACAGCCTCAAGGAACAACAACAAGTACTTCATCATCGTCATC
AAACTAATGGGGGAATAGTGATGTTTAATTAGTATATATAGGTTAATATCTTAAGTATGT
GAAGCATCATGTATAGAGCCAAGAACCTGTTAGACTAGTGTACTGAAAAGAACTCTTGCA
AAATATGTACTAAAGAGTTCCTGTAACAATGGAACTTCTGCGTTTTCTCTTGTCTTAAAG
AGCTTAAGGTTCTAGAAACAAAGTTCTTGTCCTTTCGG
   
Sequence Name 71-K106283-SALK_015182C-AD08
GenBank Accession LN486021 [GenBank]
Sequencing primer gene specific primer, AD08: 5'-CTCTGAATAAACCGAAAGGACAAG-3'
Sequence (A. th genome BLAST matches underlined) 
>71-K106283-SALK_015182C-AD08
ACTTAGCTCTTTAGACAGAGAAAACGCAGAAGTTCCATTGTTACAGGAACTCTTTAGTAC
ATATTTTGCAAGAGTTCTTTTCAGTACACTAGTCTAACAGGTTCTTGGCTCTATACATGA
TGCTTCACATACTTAAGATATTAACCTATATATACTAATTAAACATCACTATTCCCCCAT
TAGTTTGATGACGATGATGAAGTACTTGTTGTTGTTCCTTGAGGCTGTGGTACATCGGTT
CTCCTCCTTTTTCGGCATGAATTGACCGGAGCATCATACTTTCCGGCATCCAAAGGAAAG
TTGAGAACAGCTTTTCTTCCCCTGAGCTTAAAAGCTGCATAGTCGTAAGCCCTTGCAGCA
TCAATATCACTCTCAAAAGTGCCTAACCAAATCCTGGATCCTTTCTTTGCCGGATCTCGA
ATCTCTGCTGCAAATTTCCCCCATGGCCTCCTTCTCACGCCTCTGTAATGTCTCGTTTCT
TCAAACCTCTTTGTTTTTTTCCGGTTTGAGACAGTCTCAACCTCAGGTGACGTTGTTGTT
GATGATGATGATGATGATTCTGCTTCCATTTGCAGAATCTCTTGGACATAGGTGTAAACA
AATTGACGCTTAGACAACTTAATAACACATTGCGGACGTTTTTAATGTACTGGGGTGGTT
TTTCTTTTCACCAGTGAGACGGGCAACAGCTGATTGCCCTTCACCGCCGGGCCCTGAGAG
AGTTGCAGCA
Primers used for PCR 45xl - LBb3, show primer details
   
Sequence Name 75-K119133-SALK_015182C-45xl
GenBank Accession LN486142 [GenBank]
Sequencing primer gene specific primer, 45xl: 5'-CCTAGCAAGACAACCCTACAAGAC-3'
Sequence (A. th genome BLAST matches underlined) 
>75-K119133-SALK_015182C-45xl
CTCTTTTATTCGAGTGAAGTCATTATCTTCTTATCTTTTTACTCGAATGTGAATATGCAA
AATATCTTTTGATATTTAAGAGCTTACCTAGTGAGTCATTACTACTACGAAAATCATATA
TCAATCTTATCATAAAACTTTTAAGATAAAAAAAAAAAAAAAAAAACTTTTGAAAAATGG
GCTTTTAAAGACTTAAGTTCCAATTATAAACCCTAGTAGTTCAGGTCTTTTGGGTTTGGG
TTGGGGTTAGGTTTAAAATTTAAAATTTCAAAGGAACCTACGTCCTTAACCAACTCAATC
AAAATTCTGGTTAAAAAAAAAAATCCCCATTTTGTTAGCATTCAGCTTAGGATTCAAACC
AGGGGTAGCTCAGGGTTTTTTAAACTCTAGAGGAATAAAAGGGAGGTTAGGGAAATTTGT
CAGCATCATAAACAAAATCAAGTGGGCACAACTTGAAGGGCCCGGACAAAATATCTAAAG
TGGCCCCCAAAAAGGTTAAAGGGAAAAAACTGGGCCCCCCAGAGTTAACCCAAAGAGACC
CAGAGAGAGACTTTGGACCCCTCACCCAGGGCGGCTTTAACACATGTTAAGGATTCCTTT
CTTTATTTGGCCAACAATATCATCAAAACTTTTTCTTCAAACACCACTGGCAGTTTTTCT
TATTCCCCGGCCTGGCCAAAGGAAAAAAGAGAGAGGAAGAATTGGAAACTTTTGAGGAAA
GCTCTGATTTGGATGTTATACAAA
   
Sequence Name 61-K119132-SALK_015182C-R210
GenBank Accession LN485779 [GenBank]
Sequencing primer T-DNA primer, R210: 5'-CCCAGTACATTAAAAACGTCCGC-3'
Sequence (A. th genome BLAST matches underlined) 
>61-K119132-SALK_015182C-R210
ACCACAATATATCTGCGTCAATCTGGATCCGGTGTTATTTTGTTTCCCGTCGGCAAGAAC
AGGTGAACTAGGTTCCAGTTTCGGAACTGGGTTAAAGGGTTCTAGAGACCAGAGTCCGGA
GACAAAAGCAGTATCATCAAAGACAAAATCTTCAATGAAACCATCAGGGATCATCAAGTC
TTCAAATAGATGTTTCTGTATAACATCCAAATCAGAGCTTTCCTCAAAAGTCTCCATTTC
TTCCTCTCTCTTTTTTCTTTAGACAAGACAGGAGAATAAGAAAAACTGCAAGTGGTGTTT
GAAGAAAAAGTTTTGATGATATTGTTGGCTATATAAAGAAAGGAATCCTAAACATGTGTT
AACGCAGCCATGGGTGAGGTGTCAAAAGTCTCTCTCTGTGTCTCTCTGGTTTAACTCTGT
GGGGCCAAGTCTTATCCATTAAACATTTATGGAGGCAACTTAAGATATTTTGTCAGGACC
CTTCAAGTTGTGCCAACTTGATCTTGTCTATGATGCTGACAAATTTCACTAACATCCATT
TTATTCCTCTAGAGTTTAAAATACCTTGAGCTACCCATGGTTCGAATCCTAAGCTGAATG
CTAACAAAATGGTGATTATTTTTTTTAACTAGAATTTTGATTGAGTTGGTTAAGGACGTA
GGTTCATTTGAAATTTTAAATCTAAAACATAACACAAACCAAAACCAAAAAGACTTGAAC
TACTAGTGTTTATAATCGTAACTTAAGTCTTTNAAAGCCAATCTCTCAAAAGTTTTTTCT
TTTTTTTTTTAACCTAAAA

Last Updated on 06.12.2018 16:11