Sequences from confirmation amplicon


Confirmation sequences for line SALK_015394c and hit At4g13345 are shown.
Line ID SALK_015394c
Locus At4g13345 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr4:7767945
Primers used for PCR 30dr - LBb3, show primer details
   
Sequence Name 26-K110755-SALK_015394C-30dr
GenBank Accession LN485029 [GenBank]
Sequencing primer gene specific primer, 30dr: 5'-GAGACTGACAAATCTCCTTGTTCC-3'
Sequence (A. th genome BLAST matches underlined) 
>26-K110755-SALK_015394C-30dr
TCTCTTTTCTTTATAGAGAAGGAAGACATTATTAAGAAAAATCTAGATACATATATAACT
AGCCTATTAGATTATTAGAAGAGTAAAAGTCGGTGTCTATAAGTATTAAACAGTATTTTT
TGCCAAGGATCTATATATGATCTTCCAATGTCATGAAGAAGAGAGAGTCTGCTAGATTTA
AGGACTTATGGAAACCGGTACAAGCATTGACAACACTGGCTATGAAGGAATCAAGAACGG
TTCATGGTTTATCCAGTTCCGTAATGGCTGCAATCCATGGATGGCTAGATATGTGTACGG
TCTAATATTCCTCTTGGCAAATCTGCTTGCTTGGGCCTTGCGTGATTATGGCCGAGGCGC
CTTGACCGAAATGAGAAGTATGGTTACCTAAGTCTCTAGGATTTCTTACAATATTTTTTT
CAGAAAATTGATTGATTATAAGTTTATTCATTGTTGCAGAATTCAAGAATTGTAAAGAAG
GTGGAGACTGTTTAGGGACGGAAGGAGTTTTACGAGTTAGCTTCGGATGTTTTGTATCCT
TTTTTTCTTTTAATCAGTCAAATACTACAAACTATTTTCATGCCTTCAGAGAGATGTTTT
CTTTAACTATTTCTCTAGTTGTTCTATTTCATTATGTTTCTTTCAACCGTTGGGACATCA
AAGACGCATTCATCGAGAGATAAATGGCATTCAGGATGGTGGTTTGCTAAGCTTTTCATG
TTGCTTGGCTTAACCATCTTCCCCTTTTTGTTGCCTTCTTCCATTATTCAGTTTTACGGT
AAGATTAAGGCTAACCACCGTCTTCGGTTCATCGTTTTTCGATATCAAACTTCAGTTAGC
AAATTGACGCTTAGACAACTTAATAACACATTGCGGACGTTTTTAATGTACTGGG
   
Sequence Name 18-K110754-SALK_015394C-R210
GenBank Accession LN484775 [GenBank]
Sequencing primer T-DNA primer, R210: 5'-CCCAGTACATTAAAAACGTCCGC-3'
Sequence (A. th genome BLAST matches underlined) 
>18-K110754-SALK_015394C-R210
GTCATTTGCTACTGAAGTTTGATATCGAAAAACGATGAACCGAAGACGGTGGTTAGCCTT
AATCTTACCGTAAAACTGAATAATGGAAGAAGGCAACAAAAAGGGGAAGATGGTTAAGCC
AAGCAACATGAAAAGCTTAGCAAACCACCATCCTGAATGCCATTTATCTCTCGATGAATG
CGTCTTTGATGTCCCAACGGTTGAAAGAAACATAATGAAATAGAACAACTAGAGAAATAG
TTAAAGAAAACATCTCTCTGAAGGCATGAAAATAGTTTGTAGTATTTGACTGATTAAAAG
AAAAAAAGGATACAAAACATCCGAAGCTAACTCGTAAAACTCCTTCCGTCCCTAAACAGT
CTCCACCTTCTTTACAATTCTTGAATTCTGCAACAATGAATAAACTTATAATCAATCAAT
TTTCTGAAAAAAATATTGTAAGAAATCCTAGAGACTTAGGTAACCATACTTCTCATTTCG
GTCAAGGCGCCTCGGCCATAATCACGCAAGGCCCAAGCAAGCAGATTTGCCAAGAGGAAT
ATTAGACCGTACACATATCTAGCCATCCATGGATTGCAGCCATTACGGAACTGGATAAAC
CATGAACCGTTCTTGATTCCTTCATAGCCAGTGTTGTCAATGCTTGTACCGGTTTCCATA
AGTCCTTAAATCTAGCAGACTCTCTCTTCTTCATGACATTGGAAGATCATATATAGATCC
TTGGCAAAAAATACTGTTTAATACTTATAGACACCGACTTTTACTCTTCTAATAATCTAA
TAGGCTAGTTATATATGTATCTAGATTTTTCTTAATAATGTTCTTCCTTTCTCTAATAAA
GAAAAAGAGATTTTTGTTCTCTCGAACGAATGGA

Last Updated on 06.12.2018 16:11