Sequences from confirmation amplicon


Confirmation sequences for line SALK_015405c and hit At5g13260 are shown.
Line ID SALK_015405c
Locus At5g13260 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:4246273
Primers used for PCR 33fx - LBb3, show primer details
   
Sequence Name 18-K112129-SALK_015405C-33fx
GenBank Accession LN484779 [GenBank]
Sequencing primer gene specific primer, 33fx: 5'-CTCTCTGTGGAAATGGGTCTAAAG-3'
Sequence (A. th genome BLAST matches underlined) 
>18-K112129-SALK_015405C-33fx
TCTCTGGTTCGGTTCATGCGTCCCTCTTTCATTTTCTCATGGACTATACTTGTTCTTTTG
AAGGTTGAAGTTGCCATTACTATTACATTAGCCCAGCTAAGGTTGGCCAATACTACTCGA
CTGTCTGATATCGGTGAGCTTCTTCTGGACGCCAATAATTTTATGTTTTTTGTTGATAAA
AAGAGAAAATAAATTATAAGTGACTTTAGCCAGGCTTCTAATTTCACCATTGAGATAATA
GTGTACATGTTTTATTTTATTTTGTGTCGCATCTTAAATGGCTATTCTACTTTGCAATTG
ATGTTTTTTTCTGGTTGTTTTATATCAGAGTTAAAATCACCAGGTGGTCCTAAGATTACG
GAGACACTTGGTAAGTTTATTAGCTTATCTTACTGAATATTTATGTCAACATCTTGGTGT
TATGAAATATAAGATATGGAGTAAAGCATAATCCACAGAGTTACAATGACAAACTCTTGA
TACTATTATGACCATGAAAAACTTAACTTGAACGCCAGTATCCTATGTTCCAACAGTTCT
TGTTATTCTTAAGGATAATTCTGACCTTGGCCTGTGGTCCTTTGCAGAATTGAGTCAAGA
AGAGTCTGAGGATGTTCTCTTCAAGGAGGTACAGAATTACAAGCCCGTTCTCGCTTTCTC
TCANGGGCTTTTCCTTCATCTGATCCCTCCTTCATGCCGGTGTAAACAAATTGACGCTNA
GACAACTTAATAACACATTGCGGACGTTTTTAATGTACTGGGGTGGTTTTTCTTTTCACC
AGTGAGACGGGCAACAGCTGATTGCCCTTCACCGCCNGGCCCTGAGAGAGTTGCAGCAAG
CGGTCCACGCTGGTNTGCCCCAGCAGGCGAANATCCTGTTTGATGGTGGTTC
   
Sequence Name 02-K112128-SALK_015405C-R210
GenBank Accession LN484306 [GenBank]
Sequencing primer T-DNA primer, R210: 5'-CCCAGTACATTAAAAACGTCCGC-3'
Sequence (A. th genome BLAST matches underlined) 
>02-K112128-SALK_015405C-R210
TTTGTTACCCGGCATGAAGGAGGGATCAGATGAAGGAAAAGCCCATGAGAGAAAGCGAGA
ACGGGCTTGTAATTCTGTACCTCCTTGAAGAGAACATCCTCAGACTCTTCTTGACTCAAT
TCTGCAAAGGACCACAGGCCAAGGTCAGAATTATCCTTAAGAATAACAAGAACTGTTGGA
ACATAGGATACTGGCGTTCAAGTTAAGTTTTTCATGGTCATAATAGTATCAAGAGTTTGT
CATTGTAACTCTGTGGATTATGCTTTACTCCATATCTTATATTTCATAACACCAAGATGT
TGACATAAATATTCAGTAAGATAAGCTAATAAACTTACCAAGTGTCTCCGTAATCTTAGG
ACCACCTGGTGATTTTAACTCTGATATAAAACAACCAGAAAAAAACATCAATTGCAAAGT
AGAATAGCCATTTAAGATGCGACACAAAATAAAATAAAACATGTACACTATTATCTCAAT
GGTGAAATTAGAAGCCTGGCTAAAGTCACTTATAATTTATTTTCTCTTTTTATCAACAAA
AAACATAAAATTATTGGCGTCCAGAAGAAGCTCACCGATATCAGACAGTCGAGTAGTATT
GGCCAACCTTAGCTGGGCTAATGTAATAGTAATGGCAACTTCAACCTTCAAAAGAACAAG
TATAGTCCATGAGAAAATGAAAGAGGGACGCATGAACCAGAACCAGAGAGGATGAGAATA
AACCTTTAAAGAAGTTAGCTCCTTTAGACCC

Last Updated on 06.12.2018 16:11