Sequences from confirmation amplicon


Confirmation sequences for line SALK_015460c and hit At4g01990 are shown.
Line ID SALK_015460c
Locus At4g01990 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr4:871394
Primers used for PCR 73by - LBb3, show primer details
   
Sequence Name 12-K108551-SALK_015460C-73by
GenBank Accession LN484605 [GenBank]
Sequencing primer gene specific primer, 73by: 5'-ATTGCAAATGCTTCTGAGGTTTAT-3'
Sequence (A. th genome BLAST matches underlined) 
>12-K108551-SALK_015460C-73by
TCCCTATGTCAACAATTCAGCTATCTTACATGCTTCGAGCACTGAGTAAACTCGATGACA
TTGATGGAGTCAAGAAAGTCTTTGCAGAATGGGAATCGACATGCTGGACTTACGATATGC
GGATGGCGAATGTAGCGATTAGCAGCTACTTAAAGCAGAACATGTACGAAGAAGCTGAGG
CTGTTTTCAACGGCGCCATGAAAAAGTGTAAAGGTCAATTCTCAAAGGCAAGGCAGCTTT
TGATGATGCACCTGTTGAAGAACGATCAGGCAGATTTGGCTCTGAAGCATTTTGAAGCGG
CGGTTTTGGATCAAGACAAGAACTGGACTTGGAGCTCAGAGCTGATCAGTTCGTTCTTTC
TTCACTTTGAGGAAGCCAAAGACGTTGATGGAGCAGAAGAATTCTGCAAAACCTTGACGA
AATGGAGTCCCCTCAGTTCTGAAACTTACCTCAACAAATTAATAACAAATTGACGCTTAG
ACAACTTAATAACACATTGCGGACGTTTTTAATGTACTGGGGTGGTTTTTCTTTTCACCA
GTGAGACGGGCAACAGCTGATTGCCCTTCACCGCCTGGCCCTGAGAGAGTTGCAGCAAGC
GGTCCACGCTGGTTTGCCCCAGCAGGCGAAAATCCTGTTTGATGGTGGTTCCGAAATGGG
CAAAA
   
Sequence Name 02-K108550-SALK_015460C-R210
GenBank Accession LN484299 [GenBank]
Sequencing primer T-DNA primer, R210: 5'-CCCAGTACATTAAAAACGTCCGC-3'
Sequence (A. th genome BLAST matches underlined) 
>02-K108550-SALK_015460C-R210
GTCATTTGTTATAATTTGTTGAGGTAAGTTTCAGAACTGAGGGGACTCCATTTCGTCAAG
GTTTTGCAGAATTCTTCTGCTCCATCAACGTCTTTGGCTTCCTCAAAGTGAAGAAAGAAC
GAACTGATCAGCTCTGAGCTCCAAGTCCAGTTCTTGTCTTGATCCAAAACCGCCGCTTCA
AAATGCTTCAGAGCCAAATCTGCCTGATCGTTCTTCAACAGGTGCATCATCAAAAGCTGC
CTTGCCTTTGAGAATTGACCTTTACACTTTTTCATGGCGCCGTTGAAAACAGCCTCAGCT
TCTTCGTACATGTTCTGCTTTAAGTAGCTGCTAATCGCTACATTCGCCATCCGCATATCG
TAAGTCCAGCATGTCGATTCCCATTCTGCAAAGACTTTCTTGACTCCATCAATGTCATCG
AGTTTACTCAGTGCTCGAAGCATTGTAAGATAGCTTGAATTGTTGACATTAGGGTATCTT
TTCTTAAGCAAATCCCAAACTCGATAAACCTCAGAAGCATTTGCAA

Last Updated on 06.12.2018 16:11